Efficacy and mechanisms of an Angelica sinensis Cistanche Fiber Compound for constipation relief
Constipation is a prevalent ailment which might significantly impact the quality of people's life and rise some associated deseases risks.In this study,a chronic constipation mouse model was established using loperamide hydrochloride.Mice were gavaged an Angelica sinensis Cistanche Fiber Compound,comprised of Dang Gui[Angelica sinensis(Oliv.)Diels],Rou Cong Rong(Cistanche deserticola Y.C.Ma),wheat fiber,and low-molecular-weight xylan at high dosage(3.6 g·kg1,30 times the recommended human dose)and low dosage(0.6 gkg-1,5 times the recommended human dose)for 14 days.The study assessed the therapeutic efficacy of the compound by observing fecal morphology,measuring water content,and conducting small intestine motility experiments.Furthermore,enzyme-linked immunosorbent assays(ELISA)were conducted to evaluate the serum levels of gastrointestinal hormones including motilin(MTL),gastrin(GAS),the inflammatory factors interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α).Histopathological examination with H&E staining was employed to evaluate colonic tissue damage.Additionally,Western blot and immunohistochemistry experiments were conducted to examine the expression levels of aquaporin-3(AQP3)and c-Kit in the colon.The results indicated that the Angelica sinensis Cistanche Fiber Compound could improve fecal morphology,increase fecal water content,enhance small intestinal transit rates in mice.Additionally,there was a significant elevation in the serum levels of gastrointestinal hormones and a notable reduction in the levels of inflammatory factors.The improvement in colonic histopathological damage was accompanied by a marked decrease in the expression of the colonic water channel protein AQP3 and a significant increase in c-Kit expression.These results collectively suggested the presence of a dose-response relationship.These findings indicate that the Angelica sinensis Cistanche Fiber Compound effectively alleviates constipation in mice.Its action is associated with the regulation of colonic water channel protein AQP3 and c-Kit expression,along with the modulation of serum inflammatory factors and gastrointestinal hormones.This experiment was approved by the Animal Experiment Ethics Committee of Jinan University.
constipationintestinal lubrication and constipation relieffunctional foodcolontraditional Chinese medicine
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