A new hexacyclic triterpenoid with 13α,27-cyclopropane ring from Glechoma longituba
In order to study the compounds from Glechoma longituba(Nakai)Kupr.and explore the substance bases of its dissipating blood stasis,MCI,silica gel,Sephadex LH-20,ODS column chromatography and preparative thin layer chromatography were used to isolate and purify the compounds.The structures were identified by MS,1D NMR,and 2D NMR spectra analysis,etc.Eight triterpenoids were isolated from dichloromethane fraction of ethanol extract from G.longituba and identified as 2α,3α,16β-trihydroxy-13α,27-cyclours-l l-en-28-oic acid(1),28-norurs-12-ene-3β,17β-diol(2),28-norolean-12-ene-3β,17β-diol(3),oleanonic acid(4),3β,13β-dihydroxyurs-1 1-en-28-oic acid(5),uvaol(6),oleanolic acid(7),ursolic acid(8).Compound 1 is a new hexacyclic triterpene with 13α,27-cyclopropane structure,named euscaphic acid H;compounds 2 and 3 were isolated from Lamiaceae for the first time while compounds 4 and 5 were isolated from this genus.The in vitro anticoagulant activity of triterpenoids was evaluated by four coagulation indexes(activated partial thromboplastin time,APTT;thrombin time,TT;prothrombin time,PT;fibrinogen,FIB).Among them,compounds 1 and 6 showed obvious anticoagulant effects.
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