首页|基于谱效关系及活性验证的百合地黄汤抗抑郁成分研究

基于谱效关系及活性验证的百合地黄汤抗抑郁成分研究

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本研究通过谱效相关分析及活性验证探明百合地黄汤抗抑郁的药效成分。利用不同溶剂分次提取并结合HPLC和UHPLC-MS分析方法对百合地黄汤不同提取部位化学成分进行全面表征。动物实验方案经湖南中医药大学动物伦理委员会审查通过(审查号为LLBH-202104270001)，所有程序均严格按照动物使用和护理的伦理原则进行。采用旷场实验、悬尾实验、强迫游泳实验等经典行为学方法结合神经递质测定进行抗抑郁药效综合评价。整合三种相关分析方法进行谱效关联表征，以三种关联分析所得药效成分的交集为潜在药效成分，并进行了体外细胞实验验证。研究发现，王百合苷A、B、C、梓醇和异毛蕊花糖苷可能是百合地黄汤发挥抗抑郁作用的主要药效物质，同时也提示，多元化的谱效相关分析及活性验证有助于提升谱效相关分析结果的准确性。
Spectrum-effect relationship combined with bioactivity evaluation to discover the main antidepressant active components of Baihe Dihuang decoction
The study utilized spectral correlation analyses combined with bioactivity evaluation to examine the effective components of antidepressants in the Baihe Dihuang decoction.Firstly,the chemical fingerprints for different extraction parts in the Baihe Dihuang decoction were achieved using HPLC and UHPLC-MS technology.Then,in order to evaluate the antidepressant effect of Baihe Dihuang decoction,the animal experimental protocol has been reviewed and approved by Laboratory Animal Ethics Committee of Hunan University of Chinese Medicine(No.LLBH-202104270001),in compliance with the Institutional Animal Care Guidelines.We recorded the distance of autonomous movement of mice in open field experiment,the immobility time of tail suspension test,and the forced swimming.Additionally,we measured the content of neurotransmitters.Finally,Pearson analysis,grey correlation analysis,and orthogonal partial least squares regression analysis were utilized to establish the correlation between antidepressant efficacy indicators and fingerprinting.The spectrum-effect relationship results were confirmed through the in vitro activity verification.This study demonstrated that regaloside A,B,C,catalpol,and Isoacteoside might be the main antidepressant components in Baihe Dihuang decoction.Furthermore,it was found that using diverse mathematical models and bioactivity evaluation could enhance the accuracy of the spectral correlation analyses results.

Baihe Dihuang decoctiondepressionspectrum-effect relationshipactivity verificationentropy-TOPSISHPLC fingerprintmulti-index efficacy evaluation

胡超、赵洪庆、刘检、王璐、杨磊、张水寒、唐林

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湖南中医药大学第一附属医院,湖南长沙 410007

湖南中医药大学科技创新中心,湖南长沙 410208

湖南省中医药研究院,湖南长沙 410208

百合地黄汤 抑郁症 谱效关系 活性验证 熵权-TOPSIS法 HPLC指纹图谱 多指标药效评价

湖南省自然科学基金湖南省自然科学基金湖南省自然科学基金湖南省教育厅科研项目湖南省卫生健康委科研项目湖南省中医药科研项目湖南中医药大学中药粉体与创新药物省部共建国家重点实验室培育基地开放基金

2022JJ800932023JJ601322022JJ8009422B0362202213055417B20230542022FTKFJJ11

2024

10.16438/j.0513-4870.2023-1184

药学学报
中国药学会 中国医学科学院药物研究所

药学学报

CSTPCD北大核心
影响因子：1.274
ISSN：0513-4870
年,卷(期)：2024.59(5)