基于谱效关系及活性验证的百合地黄汤抗抑郁成分研究
Spectrum-effect relationship combined with bioactivity evaluation to discover the main antidepressant active components of Baihe Dihuang decoction
胡超 1赵洪庆 2刘检 1王璐 1杨磊 1张水寒 3唐林1
作者信息
- 1. 湖南中医药大学第一附属医院,湖南长沙 410007
- 2. 湖南中医药大学科技创新中心,湖南长沙 410208
- 3. 湖南省中医药研究院,湖南长沙 410208
- 折叠
摘要
本研究通过谱效相关分析及活性验证探明百合地黄汤抗抑郁的药效成分.利用不同溶剂分次提取并结合HPLC和UHPLC-MS分析方法对百合地黄汤不同提取部位化学成分进行全面表征.动物实验方案经湖南中医药大学动物伦理委员会审查通过(审查号为LLBH-202104270001),所有程序均严格按照动物使用和护理的伦理原则进行.采用旷场实验、悬尾实验、强迫游泳实验等经典行为学方法结合神经递质测定进行抗抑郁药效综合评价.整合三种相关分析方法进行谱效关联表征,以三种关联分析所得药效成分的交集为潜在药效成分,并进行了体外细胞实验验证.研究发现,王百合苷A、B、C、梓醇和异毛蕊花糖苷可能是百合地黄汤发挥抗抑郁作用的主要药效物质,同时也提示,多元化的谱效相关分析及活性验证有助于提升谱效相关分析结果的准确性.
Abstract
The study utilized spectral correlation analyses combined with bioactivity evaluation to examine the effective components of antidepressants in the Baihe Dihuang decoction.Firstly,the chemical fingerprints for different extraction parts in the Baihe Dihuang decoction were achieved using HPLC and UHPLC-MS technology.Then,in order to evaluate the antidepressant effect of Baihe Dihuang decoction,the animal experimental protocol has been reviewed and approved by Laboratory Animal Ethics Committee of Hunan University of Chinese Medicine(No.LLBH-202104270001),in compliance with the Institutional Animal Care Guidelines.We recorded the distance of autonomous movement of mice in open field experiment,the immobility time of tail suspension test,and the forced swimming.Additionally,we measured the content of neurotransmitters.Finally,Pearson analysis,grey correlation analysis,and orthogonal partial least squares regression analysis were utilized to establish the correlation between antidepressant efficacy indicators and fingerprinting.The spectrum-effect relationship results were confirmed through the in vitro activity verification.This study demonstrated that regaloside A,B,C,catalpol,and Isoacteoside might be the main antidepressant components in Baihe Dihuang decoction.Furthermore,it was found that using diverse mathematical models and bioactivity evaluation could enhance the accuracy of the spectral correlation analyses results.
关键词
百合地黄汤/抑郁症/谱效关系/活性验证/熵权-TOPSIS法/HPLC指纹图谱/多指标药效评价Key words
Baihe Dihuang decoction/depression/spectrum-effect relationship/activity verification/entropy-TOPSIS/HPLC fingerprint/multi-index efficacy evaluation引用本文复制引用
基金项目
湖南省自然科学基金(2022JJ80093)
湖南省自然科学基金(2023JJ60132)
湖南省自然科学基金(2022JJ80094)
湖南省教育厅科研项目(22B0362)
湖南省卫生健康委科研项目(202213055417)
湖南省中医药科研项目(B2023054)
湖南中医药大学中药粉体与创新药物省部共建国家重点实验室培育基地开放基金(2022FTKFJJ11)
出版年
2024
NETL
NSTL
万方数据