Determination of 19 components in Microctis Folium from different production areas based on UPLC-MS/MS
The paper is to establish an UPLC-MS/MS method for the simultaneous determination of 19 components in Microctis Folium from different production areas.The 50％methanol was used as extraction solvent.The Agilent ZORBAX SB C1 8(150 mm × 2.1 mm,1.8 pm)column was used;mobile phase was acetonitrile-0.1％acetic acid with gradient elution,flow rate was 0.3 mL·min-1,colume temperature was 30 ℃,and the injection volume was 2 μL;electrospray ionizaton source was used and detected in negative ion mode.The results showed that the established UPLC-MS/MS method could well separate the 19 components,and the methodological investigation results of 19 components were good.By means of orthogonal partial least squares discriminant analysis(OPLS-DA),28 batches of Microctis Folium samples from different production areas can be divided into three categories,Guangdong,Guangxi and Hainan are each classified into one category,and 10 signature compounds which affecting the quality differences of different production areas were screened out.The established method is accurate,reliable,sensitive and reproducible.It can provide a basis for the establishment of the quality standard of Microctis Folium,as well as for safety and quality research.
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