Identification of cajanonic acid A metabolites in rats by UPLC-Q-TOF-MS/MS
This research established a simple,rapid and sensitive ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS/MS)method to investigate the metabolic profiles of cajanonic acid A(CAA)in rats.After intragastric administration of CAA(30 mg·kg-1)to rats,the biological samples were detected by UPLC-Q-TOF-MS/MS.Relevant data was collected and processed,the accurate mass and MS2 spectra of the metabolites were compared with the parent compound.As a result,a total of 23 metabolites were detected,including 15 in urine,11 in bile,11 in feces,and 9 in plasma.The major metabolic pathways related to CAA included dehydrogenation,reduction,hydroxylation,methylation and glucuronide conjugation.This experiment was approved by Animal Ethics Committee of Guizhou Medical University(approval number:1603137).
NETL
NSTL
万方数据
