UPLC-Q-TOF-MS/MS法鉴定大鼠中树豆酮酸A代谢产物
Identification of cajanonic acid A metabolites in rats by UPLC-Q-TOF-MS/MS
程瑶 1班玉娟 2陈瑞 2张丽 2胡克荣 1黄静3
作者信息
- 1. 贵州医科大学,贵州省化学合成药物研发利用工程技术研究中心,贵州贵阳 550004;贵州医科大学药学院,贵州贵阳 550004
- 2. 贵州医科大学,贵州省化学合成药物研发利用工程技术研究中心,贵州贵阳 550004
- 3. 贵州医科大学药学院,贵州贵阳 550004
- 折叠
摘要
本研究建立一种简便、快速、灵敏度高的超高效液相色谱串联四极杆-飞行时间质谱(UPLC-Q-TOF-MS/MS)法,研究树豆酮酸A(CAA)在大鼠体内的代谢特征.通过灌胃给予大鼠CAA(30 mg·kg1)后,收集血浆、尿液、胆汁和粪便采用UPLC-Q-TOF-MS/MS检测生物样品,收集和处理相关数据,将代谢产物的精确质量数和MS2质谱与母体化合物进行比较.结果共检测到23种代谢物,其中尿液中有15种,胆汁中有11种,粪便中有11种,血浆中有9种.与CAA相关的主要代谢途径包括脱氢、还原、羟基化、甲基化和葡萄糖醛酸结合.本实验获得贵州医科大学动物伦理委员会批准(编号:1603137).
Abstract
This research established a simple,rapid and sensitive ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS/MS)method to investigate the metabolic profiles of cajanonic acid A(CAA)in rats.After intragastric administration of CAA(30 mg·kg-1)to rats,the biological samples were detected by UPLC-Q-TOF-MS/MS.Relevant data was collected and processed,the accurate mass and MS2 spectra of the metabolites were compared with the parent compound.As a result,a total of 23 metabolites were detected,including 15 in urine,11 in bile,11 in feces,and 9 in plasma.The major metabolic pathways related to CAA included dehydrogenation,reduction,hydroxylation,methylation and glucuronide conjugation.This experiment was approved by Animal Ethics Committee of Guizhou Medical University(approval number:1603137).
关键词
树豆酮酸A/UPLC-Q-TOF-MS/MS/代谢产物Key words
cajanonic acid A/UPLC-Q-TOF-MS/MS/metabolite引用本文复制引用
基金项目
国家自然科学基金(81660573)
贵州省科技计划(黔科合支撑[2017]2842)
贵州省科技计划(黔科中引地[2022]4017)
贵州省教育厅服务"四新""四化"科技攻关计划(黔教技[2022]004)
出版年
2024
NETL
NSTL
万方数据