Loong oil-lyotropic liquid crystals for the treatment of combined radiation and burn injury
Combined radiation and burn injury(CRBI)is a severe syndrome,which is induced by the simultaneous or successive radiation and burn;but no appropriate clinical therapies are available.Loong oil(LO)is a traditional Chinese medicine oil composed of the oil extracts of cuttlebone,safflower,walnut oil,and rapeseed oil,which has been demonstrated to own anti-radiation and tissue healing functions.In this study,glyceryl monostearate(GMO)was used for the preparation of lyotropic liquid crystals that loaded LO to obtain Loong oil-lyotropic liquid crystals(LOL)for the treatment of skin CRBI.The hexagonal phase structure of LOL was proved by small X-ray scattering(SAXS)analysis with an approximate√7∶√ 9∶√11 ratio of peaks and under the polarizing microscope with the birefringence phenomenon.LOL had a high LO loading capacity(4％)and good bio-adhesive force,favoring skin administration.Animal experiments were approved by the Ethics Committee of the Beijing Institute of Radiation Medicine,Academy of Military Medical Sciences and the experiments were conducted in accordance with relevant guidelines and regulations(approval number:IACUC-DWZX-2022-834).Compared to triethanolamine creams,LOL had higher skin permeability with total skin penetration within 1 h,benefiting the treatment of deep CRBI.A deep Ⅱ degree burn CRBI mouse model was established after whole-body irradiation with 5 Gy 60Co γ-ray and the next 6-second 100 ℃ burn injury.LOL promoted LO skin penetration,improved epithermal cell proliferation and the formation of hair follicles,accelerated wound healing,and inhibited scarring,which benefitted the therapy of CRBI.In this study,LO application fields are expanded and LO is a promising medication for the treatment of CRBI.
combined radiation and burn injuryLoong oillyotropic liquid crystalsionizing radiationskin wound
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