Identification and expression analysis of flavonoid O-methyltransferase gene family in Polygonum capitatum
Polygonum capitatum is a characteristic Miao medicine in Guizhou,commonly used in clinical practice to treat gastrointestinal and urinary tract infections.Research has found that it has good antibacterial and anti-inflammatory effects,and its main active ingredient is flavonoids.Lavonoid O-methyltransferase(FOMT)is a key enzyme for oxymethylation modification of flavonoid compounds.In order to understand the function and properties of FOMT protein in Polygonum capitatum,the transcriptome was sequenced by Illumina HiSeq 4000 high throughput sequencing technology.Then,the obtained transcripts were annotated and analyzed,and the whole genome of the FOMT gene family in Polygon capitalis was mined and identified.A total of 99 298 Unigenes were obtained,of which 71 514 were successfully annotated by the public database.In the genome of Polygonum capitatum,a total of 50 FOMT genes were identified.The phylogenetic tree showed that FOMT genes were divided into two subfamilies:caffeoyl CoA O-methyltransferase(CCoAOMT)and caffeic acid O-methyltransferase(COMT).Gene sequence analysis showed that the number of FOMT encoded amino acids ranged from 99 to 1 053 aa,the molecular weight ranged from 11 224.91 to 86 687.42 Da,and the isoelectric point ranged from 4.79 to 9.45.The 50 FOMT family members were hydrophobic proteins.Subcellular localization results showed that 54％of FOMT subfamily CCoAOMT and COMT members were located in cytoplasm and 28％were located in chloroplasts.The FOMT gene was tissue specific and highly expressed in the flowers of Polygonum capitatum,followed by the stems,and the least expressed in the roots.In this study,the FOMT gene family of Polygonum capitatum was identified and analyzed to provide theoretical basis for further study of FOMT function and biosynthesis of methylated flavonoids.
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