Establishment and evaluation methods of a novel animal model of liver depression transforming into fire syndrome-related depression
Through a compound induction method,combined with neurobehavioral,macroscopic characterization and objective pathological evaluation indicators,a murine depression model of liver depression transforming into fire syndrome was constructed and confirmed.The model was constructed using a combination of sleep deprivation,light exposure,and alternate-day food deprivation.Evaluation was conducted at three levels:face validity,constructs validity,and predictive validity.The establishment of the liver depression transforming into fire syndrome depression model was further validated through the counterproof of traditional Chinese medicine formulas.In terms of face validity,compared to the control group,mice in the model group exhibited typical depressive symptoms in neurobehavioral assessments;the general observation of the model group mice reveals disheveled and lackluster fur,along with delayed and easily agitated responses.Additionally,there is a substantial increase in water consumption.In the sleep phase detection of mouse,the model group showed a significant increase in the proportion of time spent in the wake phase during sleep,accompanied by a significant decrease in the proportions of time spent in both non-rapid eye movement(NREM)and rapid eye movement(REM)sleep phases.There are significant differences in physiological indicators such as average blood flow velocity,blood flow rate,tongue,urine,and claw color(r values)in the internal carotid artery.Structural validity demonstrated that levels of 5-hydroxytryptamine(5-HT),dopamine(DA),and γ-aminobutyric acid(GABA)in the hippocampus of model mice decreased significantly,while acetylcholine(ACh),tryptophan(Try),and glutamic acid(Glu)levels increased significantly.Treatment with Danzhi Xiaoyao San led to varying degrees of restoration in the aforementioned neurotransmitters.In terms of predictive validity,the antidepressant paroxetine effectively ameliorated depressive behaviors in the model mice,and the classic formula Danzhi Xiaoyao San demonstrated varying degrees of improvement in depression-related indicators.In conclusion,this study has established a novel animal model of liver-stagnation with the fire depression,thereby expanding the repertoire of traditional Chinese medicine depression models.This development contributes to the diversification of melancholic syndrome models in Chinese medicine,offering a broader spectrum of options for scientific exploration,efficacy evaluation,and drug screening in the future prevention and treatment of depression with traditional Chinese medicine.Animal experiments were conducted with the approval and supervision of the Animal Ethics Committee of Jiangxi University of Traditional Chinese Medicine(ethics number:20230313040).
depressionanimal modelliver depression transforming into fireDanzhi Xiaoyao powdercombination of disease and syndrome
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