摘要
采用硅胶、MCI、Sephadex LH-20及半制备高效液相等色谱技术进行分离纯化,并根据NMR、MS等波谱数据确定化合物结构,从湖南产山橿乙酸乙酯部位分离得到20个单体化合物,分别鉴定为:(1"S,3"S,6"R)-2',4',6'-三羟基-3'-[1"-(3"-羟基)-对薄荷烷基]-查尔酮(1)、sumadain D(2)、榭皮素(3)、儿茶素(4)、表儿茶素(5)、N-顺式阿魏酰酪胺(6)、N-反式阿魏酰酪胺(7)、N-反式-阿魏酰-3-甲氧基酪胺(8)、flavifloramides B(9)、northalifoline(10)、异落叶松脂素(11)、丁香树脂酚(12)、松脂素(13)、皮树脂醇(14)、二氢去氢二愈创木基醇(15)、4-methoxyl-denudaquinol(16)、miliusanal(17)、丁香酸(18)、脱落酸(19)、桂酸桂酯(20).化合物1为新化合物,化合物2～20首次从山橿中分离得到.抗肿瘤活性筛选结果表明,化合物1、2、3、16可以显著降低胃癌细胞MGC-803的存活能力,其IC50值分别为5.58、23.41、25.72、20.96 μmol·L-1.化合物5、20可以降低胃癌细胞MGC-803的存活能力,IC50值分别为98.83、89.26 μmol·L-1.
Abstract
The compounds were isolated and purified by silica gel,MCI,Sephadex LH-20 and semi-preparative high performance liquid chromatography.The structures of the compounds were determined by NMR and MS spectroscopic data.Twenty monomer compounds were isolated from the ethyl acetate extract of Lindera reflexa root from Hunan province,and identified as:(1"S,3"S,6"R)-2',4',6'-trihydroxy-3'-[1"-(3"-hydroxy)-p-menthanyl]-chalcone(1),sumadain D(2),quercetin(3),catechin(4),epicatechin(5),N-cis-feruloyltyramine(6),N-trans-feruloyltyramine(7),N-trans-feruloyl-3-methoxytyramine(8),flavifloramides B(9),northalifoline(10),isolariciresinol(11),syringaresinol(12),pinoresinol(13),medioresinol(14),dehydroconiferyl alcohol(15),4-methoxyl-denudaquinol(16),miliusanal(17),syringic acid(18),abscisic acid(19),(E)-cinnamyl-(E)-cinnamate(20).Compound 1 is a new compound,and compounds 2-20 have been isolated from Lindera reflexa for the first time.The results showed that compounds 1,2,3 and 16 could significantly reduce the survival ability of MGC-803 cells with IC50 values of 5.58,23.41,25.72 and 20.96 μmol·L-1,respectively.Compounds 5 and 20 can reduce the survival ability of MGC-803 cells with IC50 values of 98.83 and 89.26 μmol·L-1,respectively.
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