摘要
采用大孔吸附树脂、MCI、ToyopearlHW-40C以及硅胶等柱色谱技术并结合半制备液相对山茱萸水提物进行分离纯化,通过HRESIMS、NMR、IR、UV和ECD等方法对分离得到的化合物进行结构鉴定.从山茱萸果实中共分离得到 12 个化合物,分别鉴定为neolignan B(1)、2,3-dihydro-7-methoxy-2-(4'-hydroxy-3'-methoxyphenyl)-3a-O-β-D-xylopyranosyloxymethyl-5-benzofuranpropanol(2)、cornucadinoside A(3)、3,4-二羟基苯 乙酮(4)、没食子酸正丁酯(5)、间羟基苯甲酸(6)、奎宁酸正丁酯(7)、5-羟基麦芽酚(8)、2-糠酸(9)、5-羟甲基糠醛(10)、5-(甲氧基羰基)-2-吡咯烷酮(11)、苹果酸二甲酯(12).化合物1为新联苯类木脂素,命名为neolignan B.化合物2、4、5、7～9、11为首次从山茱萸中分离得到.
Abstract
Macroporous adsorption resin,MCI,Toyopearl HW-40C and silica gel column chromatography combined with the semi-preparative HPLC were used to isolate and purify the water extract of Cornus officinalis.And the structures of compounds were identified by HRESIMS,NMR,IR,UV and ECD.A total of twelve com-pounds were isolated from the fruits of Cornus officinalis.They were identified as neolignan B(1),2,3-dihydro-7-methoxy-2-(4'-hydroxy-3'-methoxyphenyl)-3a-O-β-D-xylopyranosyloxymethyl-5-benzofuranpropanol(2),cornuca-dinoside A(3),3,4-dihydroxyacetophenone(4),n-butyl gallate(5),3-hydroxybenzoic acid(6),n-butyl quininate(7),5-hydroxymaltol(8),2-furolic acid(9),5-hydroxymethylfurfural(10),5-(methoxycarbonyl)-2-pyrrolidone(11),and dimethyl malate(12).Compound 1 is a new biphenyl lignan,named as neolignan B.Compounds 2,4,5,7-9 and 11 were isolated from Cornus officinalis for the first time.
基金项目
中央引导地方科技发展专项资金([2016]149)
河南省科技攻关项目(212102311091)
河南省高层次人才特殊支持计划(ZYQR201810080)
NETL
NSTL
万方数据