Hemoglobin-stabilizd platinum-based polypyrrole nanoparticles and their photothermal antitumor studies
Photothermal therapy is a new type of tumor therapy that uses near-infrared laser to specifically activate the photothermal agent accumulated in the lesion site,so as to achieve thermal ablation of cancer cells.However,the long metabolic cycle and difficult clearance of photothermal agent materials in vivo are also one of the major obstacles hindering their clinical transformation.In this work,we used hemoglobin as a novel stabilizer,hexachloroplatinic acid as a novel oxidant,and pyrrole as a monomer to prepare hemoglobin-stabilized platinum-based polypyrrole nanoparticles(Hb@PtP)by a one-step oxidative polymerization method and investigated in detail to study their physicochemical properties,such as morphology and structure.After polyethylene glycol modification,the particle size of obtained Hb@PtPP was 99.08±8.3 nm and the zeta potential was-18.7±1.2 mV.Transmission electron microscopy showed that the Hb@PtPP nanoparticles exhibited irregular spherical shape and uniform dispersion in the system.Under the irradiation of 808 nm laser with different power densities,Hb@PtPP showed power density-dependent temperature-raising behavior,and CCK-8 and dead-live staining experiments confirmed that they could effectively exert the photothermal effect to kill tumor cells.Small living animal imaging results demonstrated that Hb@PtPP had good tumor targeting and retention ability,and achieved tumor growth inhibition and ablation in vivo under laser excitation.All animal experiments involved in the study were performed in accordance with the program approved by the Animal Care and Use Committee of the Chinese Academy of Medical Sciences,Beijing Union Medical College,Institute of Radiation Medicine[IRM/2-IACUC-2312-005].
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