Preparation of Salmonella typhimurium VNP20009 conjugated with imidazole-zeolite framework and its anti-tumor effect
In this study,inspired by biomimetic mineralization process,we have developed imidazole-zeolite framework(ZIF-8)conjugated VNP20009,and chemotherapy drug doxorubicin hydrochloride(DOX)was encapsulated to obtain ZD@VNP.The morphology and the combination of ZIF-8 and VNP was characterized by transmission electron microscopy and laser confocal microscopy.Fluorescence spectrophotometry was used to examine the encapsulation rate and in vitro release rate of DOX.CCK-8 and FDA/PI cell viability staining experiments were used to evaluate the ability of ZD@VNP to inhibit cell proliferation.Melanoma mouse model was established to investigate the effect of ZD@VNP to inhibit tumor growth.It was shown that ZIF-8 was evenly bounded to the surface of VNP20009,and laser confocal microscopy results also confirm the combination of ZIF-8 with VNP in ZD@VNP.The encapsulation rate of DOX in ZD@VNP was calculated to be 85.7％±3.7％,and the release of DOX under the buffer at pH 6.0 was significantly higher than that of pH 7.4.ZD@VNP treatment resulted in a greater inhibitory effect on B16F10 cell proliferation compared to DOX treatment.Animal experiment results showed that compared with VNP+DOX,ZD@VNP treatment can significantly inhibit the growth of B1 6F10 tumors in C57BL/6 mice and prolonged survival(all animal experiments were approved by the Institutional Animal Care and Ethics Committee Guangdong Medical University,No.GDMU-2023-2518).In summary,ZD@VNP was prepared through a facile,one-step method,which can significantly enhance the proliferation inhibitory effect of DOX to inhibit tumor growth and prolong survival.Our results demonstrate that ZD@VNP has great application prospects in the field of drug delivery.
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