Identification and quality evaluation of germplasm resources of commercial Acanthopanax senticosus based on DNA barcodes and HPLC
Acanthopanax senticosus is one of the genuine regional herb in Northeast China.In this study,we identified the germplasm resources of commercial A.senticosus samples based on atpIand atpB_rbcL according to the previous chloroplast genome sequencing results,and determinated the content of syringin by HPLC to evaluated the quality of commercial samples.A total of 80 A.senticosus samples were collected from 47 cities in 24 provinces.DNA was extracted to amplify the products of atpI and atpB_rbcL by PCR.The results showed that 7 haplotypes(H2,H5,H8,H10,H12,H14,H23)were formed by the combined analysis of the two gene fragments.H2 from Yichun in Heilongjiang,Shangzhi in Harbin,Suihua in Heilongjiang,Fushun in Liaoning,Benxi in Liaoning,Changbai in Jilin and Jingyu in Jilin were the dominant genotype,representing 58.75％of the total samples.H14 and H23 were the unique haplotypes of the producing area.It is speculated that the commercial A.senticosus samples with haplotypes of H14 and H23 are from Raohe,Shuangyashan,Heilongjiang and Mingshui,Suihua,Heilongjiang,respectively.HPLC analysis indicated that the content of syringin in 73.96％of the samples met the Pharmacopoeia standards.There was a significant difference in the content of syringin among the samples,ranging from 0.003 7％to 0.524 5％,with a difference of 0.520 8％,indicating that the quality of the samples in the market of A.senticosus was uneven.However,there were no significant differences in the contents of syringin in the commercial A.senticosus among different haplotypes.The content of syringin in the haplotype H23 in the market sample is relatively high,so it may be a germplasm with better quality.This study researched the germplasm resources and medicinal materials quality of commercial A.senticosus samples and will help guide the commercial circulation,reasonable medication of A.senticosus,and the screening of excellent germplasm.
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