The material basis and mechanism of action of anti-inflammatory effects of simplified Zhiqin Decoction
The anti-inflammatory effect of simplified Zhiqin Decoction was observed by using lipopolysaccharide(LPS)-induced inflammation mouse model.The main chemical constituents and the main mechanism of action of simplified Zhiqin Decoction were predicted by network pharmacology.Animal experiments verified the anti-inflammatory mechanism of simplified Zhiqin Decoction(this experiment was approved by the Animal Experiment Ethics Committee of Southwest University,approval number:IACUC-20210825-02).Simplifying Zhiqin Decoction has a significant anti-inflammatory effect on inflammatory mice,can significantly improve the overall macro shape of mice,reduce body temperature,water intake,increase the number of autonomous activities;alleviate liver,lung,spleen,thymus inflammation and pathological damage;decrease tumor necrosis factor-α(TNF-α),interleukin(IL)-1β and IL-6,nitric oxide(NO),prostaglandin E2(PGE2)content in serum and urine.The contents of serum immune factors IgG,IgA and IgM were increased.Network pharmacology predicted 66 potential anti-inflammatory active components of simplified Zhiqin Decoction involving 132 inflammatory targets,and the key anti-inflammatory signaling pathway involved PI3K/AKT,TNF,JAK-STAT,etc.Animal experiments show that simplified Zhiqin Decoction can significantly reduce the expression of JAK2/STAT-PI3K/AKT-NF-κB-TNF signaling pathway related proteins in lung tissue of inflammatory mice.The results of this study showed that simplified Zhiqin Decoction had significant anti-inflammatory effects,and its main anti-inflammatory components were quercetin,β-sitosterol,kaempferol,wogonin,stigmasterol,luteolin,neobaicalein,etc.The main mechanism of its anti-inflammatory action is that it significantly inhibits the expression of JAK2/STAT-PI3K/AKT-NF-κB-TNF signaling pathway protein.
simplified Zhiqin Decoctioninflammatory pharmacodynamicsmechanism of actionpharmaco-dynamic material basisnetwork pharmacology
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