简化栀芩汤的抗炎药效物质基础及作用机制研究
The material basis and mechanism of action of anti-inflammatory effects of simplified Zhiqin Decoction
王坤 1刘洋 1殷玥 1肖潇 1周雪娇 1袁智英 1叶良红 2徐晓玉3
作者信息
- 1. 西南大学药学院&中医药学院,重庆 400715
- 2. 重庆市铜梁区中医院,重庆 402560
- 3. 西南大学药学院&中医药学院,重庆 400715;西南大学医院,重庆 400715;国家中医药管理局高水平中药药理学重点建设学科,重庆 400715;重庆市中医康复学重点建设学科,重庆 400715
- 折叠
摘要
探讨简化栀芩汤抗炎作用的效果、主要药效成分及其作用机制.采用脂多糖(lipopolysaccharide,LPS)诱导炎症小鼠模型,观察简化栀芩汤抗炎效果;网络药理学方法预测简化栀芩汤主要化学成分及其主要作用机制;动物实验验证简化栀芩汤抗炎作用机制(本实验获得西南大学动物实验伦理委员会批准,批准号:IACUC-20210825-02).简化栀芩汤对炎症小鼠具有显著的抗炎作用,能显著改善小鼠宏观整体形态,降低体温、饮水量,增加自主活动次数;减轻肝、肺、脾、胸腺炎症病理损伤;降低血清和尿液中肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素(interleukin,IL)-1β 和 IL-6、一氧化氮(nitric oxide,NO)、前列腺素E2(prostaglandin E2,PGE2)含量;升高血清免疫因子IgG、IgA、IgM含量.网络药理学预测得到简化栀芩汤66个潜在抗炎活性成分,涉及132个炎症靶点,其抗炎关键信号通路涉及PI3K/AKT、TNF、JAK-STAT等.动物实验证明简化栀芩汤能显著降低炎症小鼠肺组织JAK2/STAT-PI3K/AKT-NF-κB-TNF信号通路相关蛋白的表达.本研究结果表明,简化栀芩汤具有显著的抗炎作用,其抗炎的主要药效成分有槲皮素、β-谷甾醇、山柰酚、汉黄芩、豆甾醇、木犀草素、黄芩新素等,其抗炎的主要作用机制是显著抑制了 JAK2/STAT-PI3K/AKT-NF-κB-TNF信号通路蛋白的表达.
Abstract
The anti-inflammatory effect of simplified Zhiqin Decoction was observed by using lipopolysaccharide(LPS)-induced inflammation mouse model.The main chemical constituents and the main mechanism of action of simplified Zhiqin Decoction were predicted by network pharmacology.Animal experiments verified the anti-inflammatory mechanism of simplified Zhiqin Decoction(this experiment was approved by the Animal Experiment Ethics Committee of Southwest University,approval number:IACUC-20210825-02).Simplifying Zhiqin Decoction has a significant anti-inflammatory effect on inflammatory mice,can significantly improve the overall macro shape of mice,reduce body temperature,water intake,increase the number of autonomous activities;alleviate liver,lung,spleen,thymus inflammation and pathological damage;decrease tumor necrosis factor-α(TNF-α),interleukin(IL)-1β and IL-6,nitric oxide(NO),prostaglandin E2(PGE2)content in serum and urine.The contents of serum immune factors IgG,IgA and IgM were increased.Network pharmacology predicted 66 potential anti-inflammatory active components of simplified Zhiqin Decoction involving 132 inflammatory targets,and the key anti-inflammatory signaling pathway involved PI3K/AKT,TNF,JAK-STAT,etc.Animal experiments show that simplified Zhiqin Decoction can significantly reduce the expression of JAK2/STAT-PI3K/AKT-NF-κB-TNF signaling pathway related proteins in lung tissue of inflammatory mice.The results of this study showed that simplified Zhiqin Decoction had significant anti-inflammatory effects,and its main anti-inflammatory components were quercetin,β-sitosterol,kaempferol,wogonin,stigmasterol,luteolin,neobaicalein,etc.The main mechanism of its anti-inflammatory action is that it significantly inhibits the expression of JAK2/STAT-PI3K/AKT-NF-κB-TNF signaling pathway protein.
关键词
简化栀芩汤/炎症药效学/作用机制/药效物质基础/网络药理学Key words
simplified Zhiqin Decoction/inflammatory pharmacodynamics/mechanism of action/pharmaco-dynamic material basis/network pharmacology引用本文复制引用
基金项目
重庆市科卫联合中医药科技项目(zy201802041)
重庆市中医康复学重点建设学科(2021-4322190044)
出版年
2024
NETL
NSTL
万方数据