绞股蓝中4个新达玛烷型三萜皂苷的分离鉴定
Four new dammarane-type triterpenoid saponins from Gynostemma pentaphyllum(Thunb.)Makino
杨光 1梁海珍 2张洁 2陈晓娟 2郭宝林 3马百平1
作者信息
- 1. 广东药科大学,广东广州 510006;军事科学院军事医学研究院辐射医学研究所,北京 100850
- 2. 军事科学院军事医学研究院辐射医学研究所,北京 100850
- 3. 中国医学科学院、北京协和医学院药用植物研究所,北京 100193
- 折叠
摘要
本文对广西产绞股蓝[Gynostemma pentaphyllum(Thunb.)Makino]中达玛烷型三萜皂苷成分进行研究,综合使用D101大孔吸附树脂、硅胶和C18柱色谱以及制备型高效液相色谱等分离手段,从中分离得到4个达玛烷型三萜皂苷类化合物,利用MS、NMR和圆二色谱(CD)等波谱技术鉴定其结构,分别为3β,12β-二羟基-25-过氧氢基达玛烷-20,23-二烯-3-O-[β-D-吡喃葡萄糖基(1→2)]-β-D-吡喃葡萄糖苷(1)、3β,12β,24S-三羟基达玛烷-20,25-二烯-3-O-[β-D-吡喃葡萄糖基(1→2)]-β-D-吡喃葡萄糖苷(2)、3β,20α-二羟基-24-烯-12β,22S-环氧达玛烷-3-O-[β-D-吡喃葡萄糖基(1→2)]-β-D-吡喃葡萄糖苷(3)、3β,12β,20S-三羟基达玛烷-24-烯-3-O-[6-O-乙酰基-β-D-吡喃葡萄糖基(1→2)-β-D-吡喃葡萄糖基]-20-O-β-D-吡喃木糖基(1→3)-α-L-吡喃鼠李糖基(1→6)-β-D-吡喃葡萄糖苷(4).化合物1～4均为新化合物.
Abstract
Damarane-type triterpene saponins are the main active ingredients in Gynostemma pentaphyllum(Thunb.)Makino.By using D101 macroporous adsorption resin and silica gel open-columns,C18 medium-pressure column chromatography,and preparative high performance liquid chromatography,we isolated four compounds from the leaves of G.pentaphyllum planted in Guangxi Zhuang Autonomous Region.Their structures were determined by comprehensive analyses of MS,NMR data and circular dichroism spectroscopy and identified as(3β,12β)-dihydroxy-25-peroxyhydrohydrodammarane-20,23-diene-3-O-[β-D-glucopyranosyl(1→2)]-β-D-glucopy-ranoside(1),(3β,12β,24S)-trihydroxydammarane-20,25-diene-3-O-[β-D-glucopyranosyl(1 →2)]-β-D-glucopy-ranoside(2),(3β,20α)-dihydroxy-24-en-12β,22SS-epoxydammarane-3-O-[β-D-glucopyranosyl(1→2)]-β-D-glucopy-ranoside(3),(3β,12β,20S)-trihydroxydammarane-24-en-3-O-[6-O-acetyl-β-D-glucopyranosyl(1→2)-β-D-glucopy-ranosyl]-20-O-β-D-xylopyranosyl(1→3)-α-L-rhamnopyranosyl(1→6)-β-D-glucopyranoside(4).Compounds 1-4 are new dammarane-type triterpene saponins.
关键词
葫芦科/绞股蓝/三萜皂苷/分离/结构鉴定Key words
Cucurbitaceae/Gynostemma pentaphyllum/triterpenoid saponin/isolation/structural elucidation引用本文复制引用
出版年
2024
NETL
NSTL
万方数据