摘要
采用大孔吸附树脂、硅胶、ODS、葡聚糖凝胶等色谱填料,运用柱色谱、半制备液相色谱等分离技术,对益母草(Leonurus japonicus Houtt.)乙醇提取物的正丁醇部位进行分离纯化,利用高分辨质谱、红外、核磁共振等波谱技术和酸水解反应鉴定化合物的结构.从益母草中分离得到6个苯乙醇苷类化合物,分别为leonoside G(1)、leonoside E(2)、leonoside B(3)、leonoside F(4)、cistanoside G(5)、红景天苷(6),其中化合物 1 为从益母草中获得的新的苯乙醇苷类化合物.
Abstract
The column chromatography and semi-preparative liquid phase chromatography with several chromatographic packing materials,including macroporous adsorbent resin,silica gel,ODS,and Sephadex LH-20,were used for the separation and purification of n-butanol-soluble portion of ethanol extract of Leonurus japonicus Houtt.The structures of isolates were identified by HR-MS,IR,NMR,and acid hydrolysis reaction.Six phenylethanol glycosides were obtained from L.japonicus and identified as leonoside G(1),leonoside E(2),leonoside B(3),leonoside F(4),cistanoside G(5),and salidroside(6).Compound 1 is a new phenylethanol glycoside.
基金项目
国家自然科学基金面上项目(81872991)
国家自然科学基金联合基金重点支持项目(U19A2010)
国家自然科学基金优秀青年科学基金项目(82022072)
NETL
NSTL
万方数据