UPLC-Q-TOF/MSE技术分析鉴定Zg02在大鼠体内的代谢产物
Identification of Zg02 metabolites in rats by UPLC-Q-TOF/MSE
张曼 1陈瑞 2胡克荣 1程瑶 1黄静3
作者信息
- 1. 贵州医科大学,贵州省化学合成药物研发利用工程技术研究中心,贵州贵阳 550004;贵州医科大学药学院,贵州贵阳 550004
- 2. 贵州医科大学,贵州省化学合成药物研发利用工程技术研究中心,贵州贵阳 550004
- 3. 贵州医科大学药学院,贵州贵阳 550004
- 折叠
摘要
本研究采用超高效液相色谱-四极杆-飞行时间-串联质谱(UPLC-Q-TOF/MSE)技术对SD大鼠经灌胃新型胰岛素增敏剂Zg02(20 mg·kg-1)后所收集的血浆、尿液及粪便样品进行检测,获得化合物的分子离子及质谱碎片离子信息,并结合UNIFI软件,对代谢产物进行快速分析.结果表明,单次灌胃Zg02(20 mg·kg-1)后在大鼠体内共推测出12个代谢产物,其中血浆、尿液、粪便中分别有5、7、11个代谢产物(含交叉分析),代谢途径主要为葡萄糖醛酸化、葡萄糖基化等结合反应.所有动物实验方案均获得贵州医科大学动物伦理委员会批准(编号:2100856).
Abstract
In this study,plasma,urine and fecal samples were collected from rats after intragastric administra-tion of novel insulin sensitizer Zg02(20 mg·kg-1).The ultra-performance liquid chromatography-quadrupole-time-of-flight-tandem mass spectrometry(UPLC-Q-TOF/MSE)techniques was used to obtain the molecular ion and mass spectrometry fragment ion information of the compound,and the metabolites were quickly analyzed by combining with UNIFI metabolite software.The results showed that a total of 12 metabolites were inferred in rats after a single gavage of Zg02(20 mg·kg-1),including 5,7 and 11 metabolites in plasma,urine and feces(including cross-analysis),and the metabolic pathways were mainly glucuronidation and glucosylation.All animal protocols were approved by the Animal Ethics Committee of Guizhou Medical University(No.2100856).
关键词
UPLC-Q-TOF/MSE/代谢产物/大鼠体内Key words
UPLC-Q-TOF/MSE/metabolite/rats in vivo引用本文复制引用
基金项目
贵州省科技计划项目(黔科中引地[2022]4017)
贵州省教育厅服务"四新""四化"科技攻关项目(黔教技[2022]004)
出版年
2024
NETL
NSTL
万方数据