The traditional commodity specifications of Chinese medicinal materials are mainly divided into different grades based on macroscopic characteristics.As the basis for high quality and good price,there is still a lack of systematic evaluation on whether they are consistent with the current standards and whether they can reflect the internal quality of medicinal material.Panax notoginseng is a commonly used,large consumption of Chinese medicinal material.At present,it is divided into 8 grades in the market based on"Tou"(the number of crude drug/500 g),but it is not related to the standard of total saponins of Panax notoginseng(the sum of three saponins)in Chinese Pharmacopoeia.In this study,ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry(UPLC-Q-TOF-MS/MS)coupled with mass spectrometry molecular network were used for the rapid identification of saponins of Panax notoginseng with different"Tou"and a total of 64 saponins were identified.Seventeen saponins related to"Tou"were screened by orthogonal partial least squares discriminant analysis(OPLS-DA).The content of five saponins R1,Rb1,Rg1,Rd,and Re in Panax notoginseng with different"Tou"was deter-mined by high performance liquid chromatography(HPLC).The results of correlation analysis showed that Rd and R,with the largest VIP values among the differential saponins,which significantly negatively correlated with"Tou"(P＜0.05).Based on the determination results of 36 batches of samples,using Rd/Total Panax notoginseng saponins(TPNS)ratio(＞0.08)as the index,Panax notoginseng can be divided into two grades:20-60"Tou"(superior)and 80-200"Tou"(qualified).Based on the concept of"macroscopic characteristics and chemical profiling",this study integrates the non-targeted analysis and quantitative determination methods to provide a new strategy for quality evaluation of Panax notoginseng.
Panax notoginsenggradequality evaluationhigh-resolution mass spectrometrysaponin
NETL
NSTL
万方数据
