基于"性状-化学成分"关联性分析的三七质量评价新策略
A new strategy for quality evaluation of Panax notoginseng based on the correlation between macroscopic characteristics and chemical profiling
王子樱 1范文翔 1刘龙婵 1陆美龙 1谷丽华 1李林楠 1杨莉 1王峥涛1
作者信息
- 1. 上海中医药大学中药研究所,中药标准化教育部重点实验室,国家中医药管理局中药新资源与质量评价重点实验室,上海 201203;上海中药标准化研究中心,上海 201203
- 折叠
摘要
传统的中药材商品规格主要依据性状等感官指标划分为不同的等级,作为优质优价的依据,与现行标准是否吻合、是否能够反映药材的内在质量,尚缺乏系统评价.三七为常用、大宗药材,目前市场上仅根据头数(支数/500 g)划分为8个等级,但与《中华人民共和国药典》规定的以三七总皂苷(3种皂苷之和)含量为指标的标准不相关.本研究采用超高效液相色谱-四级杆-飞行时间串联质谱(UPLC-Q-TOF-MS/MS)并结合质谱分子网络对不同头数三七皂苷类成分进行快速鉴定,共表征64种皂苷成分.通过正交偏最小二乘判别分析(OPLS-DA)筛选出与三七头数相关的17个皂苷差异化合物;采用高效液相色谱法(HPLC)对不同头数三七中5种主要皂苷类成分R1、Rb1、Rg1、Rd、Re进行含量测定,相关性分析结果表明Rd、R1是差异皂苷中VIP值最大的皂苷类成分,与三七头数呈显著负相关(P＜0.05).基于36批三七样品测定结果,采用Rd/三七总皂苷(TPNS)比值(＞0.08)为指数,可将三七划分为20～60头(优选)与80～200头(统货)两个等级.本研究基于"性状-化学分析"相结合的理念,整合非靶向定性分析与定量测定方法,为三七品质评价提供新的科学依据和策略.
Abstract
The traditional commodity specifications of Chinese medicinal materials are mainly divided into different grades based on macroscopic characteristics.As the basis for high quality and good price,there is still a lack of systematic evaluation on whether they are consistent with the current standards and whether they can reflect the internal quality of medicinal material.Panax notoginseng is a commonly used,large consumption of Chinese medicinal material.At present,it is divided into 8 grades in the market based on"Tou"(the number of crude drug/500 g),but it is not related to the standard of total saponins of Panax notoginseng(the sum of three saponins)in Chinese Pharmacopoeia.In this study,ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry(UPLC-Q-TOF-MS/MS)coupled with mass spectrometry molecular network were used for the rapid identification of saponins of Panax notoginseng with different"Tou"and a total of 64 saponins were identified.Seventeen saponins related to"Tou"were screened by orthogonal partial least squares discriminant analysis(OPLS-DA).The content of five saponins R1,Rb1,Rg1,Rd,and Re in Panax notoginseng with different"Tou"was deter-mined by high performance liquid chromatography(HPLC).The results of correlation analysis showed that Rd and R,with the largest VIP values among the differential saponins,which significantly negatively correlated with"Tou"(P＜0.05).Based on the determination results of 36 batches of samples,using Rd/Total Panax notoginseng saponins(TPNS)ratio(＞0.08)as the index,Panax notoginseng can be divided into two grades:20-60"Tou"(superior)and 80-200"Tou"(qualified).Based on the concept of"macroscopic characteristics and chemical profiling",this study integrates the non-targeted analysis and quantitative determination methods to provide a new strategy for quality evaluation of Panax notoginseng.
关键词
三七/等级/品质评价/高分辨质谱法/皂苷Key words
Panax notoginseng/grade/quality evaluation/high-resolution mass spectrometry/saponin引用本文复制引用
基金项目
国家自然科学基金委员会重点项目(82130115)
上海市中医药三年行动计划项目(ZY2021-2023-0215)
出版年
2024
NETL
NSTL
万方数据