A new strategy for quality evaluation of Panax notoginseng based on the correlation between macroscopic characteristics and chemical profiling
The traditional commodity specifications of Chinese medicinal materials are mainly divided into different grades based on macroscopic characteristics.As the basis for high quality and good price,there is still a lack of systematic evaluation on whether they are consistent with the current standards and whether they can reflect the internal quality of medicinal material.Panax notoginseng is a commonly used,large consumption of Chinese medicinal material.At present,it is divided into 8 grades in the market based on"Tou"(the number of crude drug/500 g),but it is not related to the standard of total saponins of Panax notoginseng(the sum of three saponins)in Chinese Pharmacopoeia.In this study,ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry(UPLC-Q-TOF-MS/MS)coupled with mass spectrometry molecular network were used for the rapid identification of saponins of Panax notoginseng with different"Tou"and a total of 64 saponins were identified.Seventeen saponins related to"Tou"were screened by orthogonal partial least squares discriminant analysis(OPLS-DA).The content of five saponins R1,Rb1,Rg1,Rd,and Re in Panax notoginseng with different"Tou"was deter-mined by high performance liquid chromatography(HPLC).The results of correlation analysis showed that Rd and R,with the largest VIP values among the differential saponins,which significantly negatively correlated with"Tou"(P＜0.05).Based on the determination results of 36 batches of samples,using Rd/Total Panax notoginseng saponins(TPNS)ratio(＞0.08)as the index,Panax notoginseng can be divided into two grades:20-60"Tou"(superior)and 80-200"Tou"(qualified).Based on the concept of"macroscopic characteristics and chemical profiling",this study integrates the non-targeted analysis and quantitative determination methods to provide a new strategy for quality evaluation of Panax notoginseng.
Panax notoginsenggradequality evaluationhigh-resolution mass spectrometrysaponin
NETL
NSTL
万方数据
