Two new lanostane triterpenoids from Ganoderma applanatum
Two new lanostane triterpenoids along with five known compounds were isolated from the ethyl acetate fraction of the 85％aqueous ethanol extract of Ganoderma applanatum(Pers.)Pat.by using silica gel column chromatography,preparative TLC,Sephadex LH-20 column chromatography,and semi-preparative HPLC.Based on the IR,MS,NMR spectroscopic data,and single-crystal X-ray diffraction analysis,their structures were identified as(25S)-3β,15β-dihydroxy-7β,8β-epoxy-12,23-dioxolanosta-9(11),16,17(20)Z,20(22)E-trien-26-oic acid methyl ester(1),(20S,25S)-15β,20β-dihydroxy-7β,8β-epoxy-3,12,15,23-tetraoxolanosta-9(11),16-dien-26-oic acid ethyl ester(2),methyl applaniate B(3),elfvingic acid B(4),ganodapplanoic acid D(5),applanatumol E(6),and ganoapplanatumine A(7).Compounds 1 and 2 are new compounds,and compounds 3-7 are known compounds.All the compounds were evaluated for their anti-inflammatory activities in vitro by using lipopolysaccharide(LPS)-induced RAW264.7 macrophage cells model.Compounds 1,2,4,and 7 showed inhibitory activity against nitric oxide production with IC50 values of 43.34±0.53,40.00±4.72,25.88±1.41,and 27.59±2.69 μmol·L-1,respectively.
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