Extracting extracts of secondary metabolites from the karst cave fungus Metarhizium anisopliae NHC-M3-2 from the Yilingyan Scenic Area in Guangxi.Ten compounds were isolated and purified from fungal secondary metabolites using thin-layer chromatography and high-performance liquid chromatography.7-Hydroxy-3-hydroxypropyl-5,6-dimethylisochrome-1-one(1)and 7-hydroxy-3,5,6-trimethyl-isochromen-1-one(2)were new isocoumarin compounds,N-acetyl phenylalanine(3),chaetosumin J(4),2-one-13-hydroxy-3,5,8,7(11)-eudesmatet-raen-12,8-olide(5),1H-indole-3-carboxaldehyde(6),irpexolaceus B(7),irlactin L(8),cytochalasin K(9),and helvolic acid(10),a total of 8 known compounds.The structure of the compound was determined using methods such as NMR and mass spectrometry.The tumor cytotoxicity of the compound was evaluated using the CCK-8 method.The results showed that the IC50of compound 2 on HepG2 cells was 29.83 μmol·L-1,and compound 9 on HCT116 cells was 27.44 μmol·L-1.
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