Study on multi-component contents of Jinqi Jiangtang Capsule
Jinqi Jiangtang Capsule(JQJTC)is clinically used for the prevention and treatment of type 2 diabetes,but the contents of its main chemical components are not yet clear.In this study,an ultra-high-performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS)method was established for the determination of 15 components in JQJTC,including new chlorogenic acid,chlorogenic acid,cryptochlorogenic acid,formononetin,ononin,calycosin,calycosin-7-glucoside,astragaloside IV,berberine,epiberberine,berberrubine,coptisine,jatror-rhizine,palmatine and magnoflorine.The method was used to determine the contents of 15 components in the capsule and then to investigate the influence of excipients on the contents of the components in JQJTC.The separa-tion was performed on a ACQUITY UPLC BEH C18 column(100 mm × 2.1 mm,1.7 μm)with a mobile phase consisting of 0.1％acetic acid and 5 mmol·L-1 ammonium acetate(A)and acetonitrile(B)with gradient elution at a flow rate of 0.3 mL·min-1 and a column temperature at 40 ℃.Electron spray ionization was used for mass spec-trometry in positive ion mode.The established method meets the requirements of methodology of content determi-nation in Chinese pharmacopoeia.The contents of 15 components in JQJTC varied from high to low.The top 5 contents were berberine,chlorogenic acid,magnoflorine,coptisine,and cryptochlorogenic acid,accounting for 87.31％of the total content.The contents of 10 components,including the alkaloids of coptidis rhizoma(berberine,epiberberine,berberrubine,coptisine,jatrorrhizine,palmatine and magnoflorine)and the organic acids of honey-suckle(new chlorogenic acid,chlorogenic acid,and cryptochlorogenic acid)in the whole formula extract without excipients was significantly lower than that in the capsule.These components accounted for 99.20％of the deter-mined component contents.In this experiment,an accurate,sensitive and efficient UHPLC-MS/MS method for the determination of multi-components in JQJTC was established,which stably and reliably detected the contents of 15 components in the capsule and could provide the basis for more comprehensive quality analysis.It was also found that excipients had an increasing effect on the contents of detected alkaloid and organic acid components,which may be beneficial to the effectiveness of the capsules.
Jinqi Jiangtang Capsulemulti-component contentexcipientultra performance liquid chroma-tography-tandem mass spectrometry
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