络石内生菌Myrothecium roridum产生的一个新单端孢霉烯大环内酯
A new trichothecene macrolide from endophyte Myrothecium roridum associated with Trachelospermum jasminoides
韩旭 1李晓雯 2张凤 2方蕾 2申丽1
作者信息
- 1. 扬州大学医学院,江苏扬州 225009;江苏省中西医结合老年病防治重点实验室,江苏扬州 225009
- 2. 扬州大学医学院,江苏扬州 225009
- 折叠
摘要
采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱以及高效液相色谱法从络石(Trachelospermum jasminoides)内生菌Myrothecium roridum IFB-E008发酵液的粗浸膏中分离得到3个化合物.经高分辨质谱、一维和二维核磁共振谱及文献比对等方法,鉴定为:3'-iso-isororidinA(1)、verrol(2)和N-乙酰色胺(3),其中,3'-iso-isororidin A(1)是一个未见文献报道的新单端孢霉烯大环内酯.体外细胞毒活性测定表明,化合物2对人胃癌细胞株SGC-7901具有一定的抑制活性,半数抑制浓度(half inhibition concentration,IC50)为 59.79 μg·mL-1(158.1 μmol·L-1),阳性对照顺铂的IC5.值为 6.58 μg·mL-1(21.9 μmol·L-1).
Abstract
Three compounds were obtained from the crude extract of the fermentation broth of endophyte Myrothecium roridum IFB-E008 in Trachelospermum jasminoides by silica gel column chromatography,Sephadex LH-20 gel column chromatography and high performance liquid chromatography.They were determined as 3'-iso-isororidin A(1),verrol(2)and N-acetyltryptamine(3),respectively,through HR-MS,1D and 2D NMR and literature comparison.3'-iso-isororidin A(1)is a new trichothecene macrolide that has never been reported in the literature.The in vitro cytotoxicity assay showed that compound 2 had certain cytotoxicity against the human gastric cell line SGC-7901 with half inhibition concentration(IC50)of 59.79 μg·mL-1(158.1 μmol·L-1),while IC50 value of the positive control cisplatin was 6.58 μg·mL-1(21.9 μmol·L-1).
关键词
络石/内生菌/Myrothecium/roridum/次生代谢产物/单端孢霉烯Key words
Trachelospermum jasminoides/endophyte/Myrothecium roridum/secondary metabolite/trichot-hecene引用本文复制引用
基金项目
国家自然科学基金项目(21372191)
扬州市重点研发项目(社会发展)(YZ2023068)
出版年
2024
NETL
NSTL
万方数据