摘要
采用ODS、MCI gel CHP20以及硅胶等柱色谱技术并结合半制备液相对茯苓95％乙醇提物进行分离纯化,运用NMR、MS、IR、计算NMR等方法,对分离得到的化合物进行结构鉴定.从茯苓中共分离得到7个化合物,分别鉴定为20S-2β,3α,15α,19,20-羟基-孕甾-7-烯(1)、去氢齿孔酸乙酰酯(2)、齿孔酸乙酰酯(3)、去氢齿孔酸(4)、齿孔酸(5)、去氢茯苓酸(6)、茯苓酸(7).化合物1为新孕甾烷类化合物.
Abstract
The 95％ ethanol extract of Poria cocos was separated and purified by ODS,MCI gel CHP20 and silica gel column chromatography combined with the semi-preparative HPLC.The chemical structures of the isolated compounds were identified by NMR,MS,IR,and calculated NMR methods.Seven compounds were isolated from Poria cocos and identified as 20S-2β,3α,15α,19,20-hydroxy-pregnane-7-ene(1),dehydroeburicoic acid monoacetate(2),eburicoic acid acetate(3),dehydroeburicoic acid(4),eburicoic acid(5),dehydropachymic acid(6),pachymic acid(7).Compound 1 is a new pregnane steroid.
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