The methanol extract of Huperzia serrata was separated and purified by ODS,AB-8 macroporous adsorption resin,dextran gel Sephadex LH-20 and silica gel column chromatography combined with the semi-pre HPLC.The chemical structures of the isolated compounds were identified by MS,IR,NMR,etc.Four compounds were isolated from Huperzia serrata and identified,named as serratinine C(1),lycobeline C(2),diphaladin A(3)and crenatine(4).Compound 1 is a new alkaloid,compounds 2-4 were isolated for the first time.In vitro biological activity experiments showed that compound 1 could significantly reduce the levels of nitric oxide and reactive oxygen species in glial cells induced by lipopolysaccharide and had significant antioxidant biological activity.
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