目的 基于数据挖掘和网络药理学研究儿童腺样体肥大的用药规律及其作用机制，为中药的临床应用提供参考。方法 检索CBM、CNKI、Wanfang、VIP中运用中药复方治疗儿童腺样体肥大的文献，对纳入处方中药物的基本应用规律及关联规则、聚类、复杂网络进行综合分析。将文献数据挖掘获得的中药治疗腺样体肥大的核心药物导入TCMSP，筛选后得到核心药物的有效成分及相应靶点。在GeneCards、OMIM和TTD数据库中检索"adenoid hypertrophy"，得到腺样体肥大的靶点;将疾病和药物靶点导入Cytoscape和STRING平台，构建"疾病-药物-成分-靶点"网络与核心药物治疗儿童腺样体肥大的PPI网络;运用DAVID数据库进行GO富集分析及KEGG通路富集分析;利用PyMol和Auto Dock Tools处理后对关键成分和靶点进行分子对接验证。结果 共纳入69篇文献，87个处方，174味中药;综合分析得到甘草、辛夷、白芷、夏枯草、浙贝母为治疗儿童腺样体肥大的核心药物。网络药理学分析显示，核心药物发挥治疗作用可能基于151个有效成分，核心药物与疾病共有109个关键治疗靶点;核心药物的有效成分主要有豆甾7-烯醇、β-谷甾醇、豆甾醇等甾醇类化合物及木犀草素、山奈酚和异鼠李素等黄酮类化合物，这些成分主要具有抗炎、抗氧化、免疫调节及清除自由基等作用，可能通过调控PI3K-AKT及TNF等关键炎症信号通路来发挥治疗作用。分子对接发现关键成分与靶点间均具有较好的结合力。结论 中药治疗儿童腺样体肥大以清热、解表、补益、化痰止咳平喘和活血化瘀药为主，用药多归肺、肝、胃经，辛夷-白芷和苍耳子-夏枯草、辛夷是关键药对，可以作用于PI3K-AKT及TNF等通路来治疗腺样体肥大，可能成为中药治疗腺样体肥大的方向之一。
Medication Rule and Mechanism of Traditional Chinese Medicine in the Treatment of Adenoidal Hypertrophy in Children Based on Data Mining and Network Pharmacology
Objective To study the medication rule and mechanism of drug use in children with adenoid hypertrophy based on data mining and network pharmacology,and provide reference for clinical application of traditional Chinese medicine.Methods Literatures on the treatment of adenoidal hypertrophy in children with traditional Chinese medicine compound in CBM,CNKI,Wanfang and VIP were retrieved,and the basic application rules,association rules,clustering and complex networks of the drugs included in the prescriptions were comprehensively analyzed.The core drugs of traditional Chinese medicine for adenoidal hypertrophy obtained by literature data mining were introduced into TCMSP,and the effective components and corresponding targets of the core drugs were obtained after screening.The"adenoid hypertrophy"was searched in GeneCards,OMIM and TTD databases to obtain the target of adenoid hypertrophy.The disease and drug targets were imported into Cytoscape and STRING platforms to construct a"disease-drug-component-target"network and a PPI network for the treatment of adenoidal hypertrophy in children with core drugs.GO enrichment analysis and KEGG pathway enrichment analysis were performed using DAVID database.After treatment with PyMol and Auto Dock Tools,the key components and targets were verified by molecular docking.Results A total of 69 articles,87 prescriptions and 174 traditional Chinese medicines were included.Comprehensive analysis showed that Gancao,Xinyi,Baizhi,Xiakucao,and Zhebeimu were the core drugs for the treatment of adenoidal hypertrophy in children.Network pharmacology analysis showed that the therapeutic effect of core drugs might be based on 151 active ingredients,and there were 109 key therapeutic targets for core drugs and diseases.The effective components of the core drugs mainly included sterols such as stigmasterol 7-enol,β-sitosterol,stigmasterol and flavonoids such as luteolin,kaempferol and isorhamnetin.These components mainly had anti-inflammatory,antioxidant,immunomodulatory and free radical scavenging effects,and might play a therapeutic role by regulating key inflammatory signaling pathways such as PI3K-AKT and TNF.Molecular docking showed that there was a good binding force between the key components and the target.Conclusion The treatment of adenoid hypertrophy in children with traditional Chinese medicine is mainly based on clearing away heat,diaphoresis relieving superficies,invigorating therapy,phlegm-eliminating cough-relieving and asthma-relieving,and blood circulation drugs.The drugs are mostly used in the lung,liver and stomach meridians.Xinyi-Baizhi and Cangerzi-Xiakucao are the key drug pairs,they can act on PI3K-AKT and TNF pathways to treat adenoid hypertrophy,which may become one of the directions of traditional Chinese medicine in the treatment of adenoid hypertrophy.
Adenoid hypertrophy in childrenTraditional Chinese medicineData miningNetwork pharmacologyMolecular docking
万方数据
维普
