摘要
目的 研究HIV/TB双重感染者血清中NLRP3炎症小体及相关细胞因子的表达水平.方法 收集2021年1月-12月喀什地区第一人民医院收治的HIV、TB、HIV/TB患者为研究对象,分别设为HIV组、TB组、HIV/TB组,每组20例,使用酶联免疫吸附法检测患者血清IL-6、IL-17、IL-22、IL-23、TNF-α的表达水平,使用实时荧光定量PCR检测患者血清中NLRP3mRNA的表达水平.比较各组实验室指标.结果 HIV/TB组IL-6、IL-17、IL-22、NLRP3mRNA水平高于TB组,IL-23水平低于TB 组,差异有统计学意义(P＜0.05);HIV/TB 组 IL-6、IL-17、IL-22、IL-23、TNF-α 及 NLRP3mRNA 水平高于 HIV 组,差异有统计学意义(P＜0.05).结论 HIV/TB双重感染患者较单纯HIV患者、TB感染者体内体内细胞因子失衡更加明显,可能与疾病的发生发展密切相关.
Abstract
Objective To investigate the expression levels of NLRP3 and related cytokines in the serum of HIV/TB co-infected patients.Methods HIV,TB and HIV/TB patients admitted to the First People's Hospital of Kashgar Prefecture from January to December 2021 were collected as the research objects,which were set as HIV group,TB group and HIV/TB group,with 20 patients in each group.The expression levels of IL-6,IL-17,IL-22,IL-23 and TNF-α in serum of patients were detected by enzyme-linked immunosorbent assay,and the expression levels of NLRP3mRNA in serum of patients were detected by real-time fluorescence quantitative PCR.The laboratory indexes of each group were compared.Results The levels of IL-6,IL-17,IL-22 and NLRP3mRNA in HIV/TB group were higher than those in TB group,and the level of IL-23 was lower than that in TB group,the difference was statistically significant(P＜0.05).The levels of IL-6,IL-17,IL-22,IL-23,TNF-α and NLRP3 mRNA in HIV/TB group were higher than those in HIV group,,the difference was statistically significant(P＜0.05).Conclusion The imbalance of cytokines in patients with HIV/TB co-infection is more obvious than that in HIV patients and TB patients,which may be closely related to the occurrence and development of the disease.
基金项目
"珠江学者·天山英才"合作专家工作室创新团队计划项目(KDYY202016)
省部共建中亚高发病成因与防治国家重点实验室开放课题项目(SKL-HIDCA-2020-10)
省部共建中亚高发病成因与防治国家重点实验室开放课题项目(SKL-HIDCA-2020-KS1)
维普
万方数据