Establishment and Comparison of Animal Models of Diabetes Mellitus Induced by Streptozocin and Special Diet
Objective To compare the establishment and effects of two different animal models of diabetes mellitus.Methods Forty male ICR mice were randomly divided into normal control group and different doses of streptozotocin groups(120,130 and 140 mg/kg).Twenty healthy male mice of clean grade were divided into normal diet control group and high-fat diet group.The blood glucose levels of normal mice and different doses of STZ groups were compared.The body weights of normal diet group and high-fat diet group were fed for 1,4,8,12 and 16 weeks.The effect of high-fat diet on glucose tolerance of diabetic mellitus model animals was analyzed.Results There was a statistically significant difference in fasting blood glucose levels between the high-dose STZ group and the normal control group(P＜0.05).The body weight of the high-fat diet group was higher than that of the normal diet group on the 4th,8th and 12th weeks of high-fat diet feeding,and the difference was statistically significant(P＜0.05).The body weight of the 16th week was higher than that of the normal diet group,but the difference was not statistically significant(P＞0.05).After 16 weeks of feeding,the blood glucose levels in the high-fat group were higher than those in the normal diet group at 30,60,and 120 min after glucose administration,and the difference was statistically significant(P＜0.05).Conclusion The animal model of diabetes mellitus induced by streptozotocin can effectively simulate the course and pathogenesis of type 1 diabetes mellitus,while the animal model of type 2 diabetes mellitus induced by special diet is suitable for preclinical screening of hypoglycemic drugs.
StreptozocinDiabetes mellitusBlood glucoseHigh-fat diet inductionAnimal model
万方数据
维普
