Expression of Exosomal Genes in Melanoma and Construction of ceRNA Network
Objective To investigate the novel molecular mechanism of melanoma(MEL)exosomes by analyzing differentially expressed genes and constructing a competing endogenous RNA(CeRNA)network.Methods The RNA expression profiles(including mRNA,lncRNA and circRNA)of peripheral blood from 21 melanoma patients and 118 healthy people were downloaded from the exoRBase 2.0 exosome database.The differentially expressed genes were identified by R's limma package and Cytoscape software.After predicting miRNAs,the CeRNA network was constructed and visualized,and the mRNAs in the CeRNA network were subjected to GO enrichment analysis.Results A total of 138 differentially expressed mRNAs,135 differentially expressed lncRNAs and 33 differentially expressed circRNAs were screened.Through the construction of CeRNA network,it was found that mRNA,lncRNA and circRNA competitively bind to miRNA,and the expression of circRNA combined with miRNA was down-regulated.The expression of mRNA was mostly down-regulated,while the expression of lncRNA was mostly up-regulated.GO enrichment analysis of differentially expressed mRNAs in the CeRNA network showed that these genes were mainly related to the regulation of mRNA catabolic processes.Conclusion There are significant differences in peripheral blood exosomes between healthy people and MEL patients,and mRNA,lncRNA and circRNA can competitively bind to MEL-related miRNAs to achieve mutual regulation.These CeRNA genes provide a molecular basis for further study of the occurrence and development of MEL,and provide new targets for early detection and treatment of MEL.
万方数据
维普
