Clinical Observation and Network Pharmacology Study on the Treatment of Rheumatoid Arthritis with Sanbi Granule
Objective To observe the clinical efficacy of Sanbi Granules in the treatment of rheumatoid arthritis(RA),and to explore its mechanism of action by network pharmacology.Methods A total of 96 patients with RA treated in the Loudi Hospital of Traditional Chinese Medicine from January 2021 to December 2022 were selected and randomly divided into study group(48 patients)and control group(48 patients).The control group was given conventional drug treatment,and the research group was given Sanbi granules on the basis of this treatment.The clinical efficacy and serum indexes[erythrocyte sedimentation rate(ESR),C-reactive protein(CRP),rheumatoid factor(RF),anti-cyclic citrullinated peptide antibody(ACPA)and interleukin-6(IL-6)]were compared between the two groups after 2 months of treatment.Based on the network pharmacological method,first,the effective components and targets of Sanbi granules were screened with TCMSP database,the key core targets were obtained by intersection of the results and the anti-RA related targets retrieved from Gene Cards databases.Secondly,STRING database was used for protein interaction analysis,protein interaction and active components-targets-pathway network was constructed by Cytoscape 3.6.1.Finally,gene ontology(GO)function and KEGG pathway enrichment analysis were performed by DAVID database,and the above results were verified by molecular docking technique.Results The total effective rate of the treatment group was 96.50％,which was higher than 68.75％of the control group(P＜0.05).The levels of ESR,CRP,RF,ACPA and IL-6 in the treatment group were better than those in the control group 2 months after treatment(P＜0.05).Through network pharmacology analysis,21 key active components and 27 potential targets were obtained,which mainly played an anti-RA role through 10 key signaling pathways.Conclusion Sanbi granules have the characteristics of multi-component,multi-target and multi-pathway synergistic intervention in the treatment of RA,which can significantly improve the clinical symptoms of RA patients and the clinical effect is remarkable.
万方数据
维普
