The Mechanism of Huangqi-Danshen in the Treatment of Liver Fibrosis Based on Network Pharmacology and Molecular Docking
Objective To study the mechanism of Huangqi-Danshen in the treatment of liver fibrosis by network pharmacology and molecular docking.Methods The TCMSP database platform was used to screen the effective components and related targets of Huangqi and Danshen.GeneCards and OMIM database platforms were used to screen the targets of liver fibrosis.Cytoscape software was used to construct the"drug-component-disease-target"network diagram,and String and David databases were used to analyze the PPI network,GO biological process and KEGG pathway of the targets.Molecular docking of components and targets was performed by AutoDock Vina.Results A total of 76 active ingredients and 64 targets corresponding to liver fibrosis were obtained from Huangqi-Danshen.There were 102 GO biological processes and 155 signaling pathways.The main signaling pathways involved were AGE-RAGE signaling pathway,IL-17 signaling pathway,TNF signaling pathway,and hypoxia-inducible factor 1(HIF-1)signaling pathway in diabetic complications.Molecular docking showed that quercetin,luteolin,kaempferol and tanshinone Ⅱ A in Radix Astragali and Radix Salviae Miltiorrhizae had good affinity with core targets AKT1,TP53,VEGFA,TNF and IL-6.Conclusion The mechanism of Huangqi-Danshen in the treatment of liver fibrosis has the characteristics of"multi-component,multi-target and multi-signal pathway",which provides a reference for future experimental research.
万方数据
维普
