Experimental Study on Irritation and Allergy of Crossbow Medicine
Objective To evaluate the skin irritation and skin allergy of crossbow medicine,and to provide a safety basis for the application of crossbow medicine.Methods Skin irritation test:four rabbits,half male and half female.Using self-control,the left side was the control site(warm water),and the right side was the administration site(crossbow medicine).After 14 days of continuous administration,the local erythema and edema were observed,and the skin irritation reaction and stimulation intensity were scored.The skin of the administration site was taken for histopathological examination.Skin allergy test:thirty guinea pigs,half male and half female,were divided into control group(warm water),2-4-dinitrochlorobenzene positive group(sensitization 1.0％0.2 ml/guinea pig,challenge 0.1％0.2 ml/guinea pig)and administration group(appropriate amount of crossbow liquid).Guinea pigs were sensitized on the left side on the 1st,7th and 14th day,and challenged on the right side on the 14th day after the last sensitization.Allergic reactions were observed at 1 h,24 h,48 h and 72 h after challenge,and the average reaction and sensitization rate of guinea pigs in each group were calculated.Results In the skin irritation test of crossbow medicine,the mean value of skin reaction score was 0,the intensity of skin irritation was non-irritation,and no histopathological changes were found in pathological examination.In the skin allergy test of guinea pigs,no erythema and edema were observed in the skin of guinea pigs in the crossbow medicine group and the control group within 72 h,and the average reaction level was 0,and the sensitization rate was 0.Conclusion In the rabbit skin irritation test and guinea pig skin allergy test,the crossbow medicine has no irritation to rabbit skin and no allergy to guinea pig skin.
万方数据
维普
