Expression of CPSF6 in Advanced Non-small Cell Lung Cancer and its Relationship with Prognosis
Objective To investigate the expression and clinical value of cleavage and polyadenylation specific factor 6(CPSF6)in advanced non-small cell lung cancer.Methods TIMER2.0 was used to analyze the expression differences of CPSF6 in tumor tissues and adjacent tissues.The visualization platform GEPIA of TCGA database was used to evaluate the effect of CPSF6 on overall survival in the context of pan-cancer.The GEPIA2 tool was used to compare the expression of CPSF6 in different pathological stages(stages Ⅰ-Ⅳ)of lung cancer.The data sets of lung adenocarcinoma and lung squamous cell carcinoma in TCGA database were retrieved online,and survival and prognosis analysis were performed.The tumor tissues of 74 patients with advanced non-small cell lung cancer admitted to our hospital from January 2018 to March 2020 and 20 lung tissues were collected for CPSF6 immunohistochemical detection.Kaplan-Meier survival analysis was used to evaluate the relationship between CPSF6 expression and progression-free survival and overall survival in patients with advanced non-small cell lung cancer.Univariate and multivariate analysis was used to evaluate the prognostic significance of CPSF6 in patients with lung cancer.Results TIMER 2.0 and TCGA database analysis showed that the level of CPSF6 mRNA in tumor tissues of patients with non-small cell lung cancer was higher than that in adjacent tissues(P＜0.05).CPSF6 was associated with poor prognosis in patients with various cancers,including lung adenocarcinoma.The expression of CPSF6 in advanced non-small cell lung cancer was higher than that in lung tissue,and the difference was statistically significant(P＜0.05).The increased expression of CPSF6 was correlated with tumor size,lymph node metastasis and clinical stage.Kaplan-Meier survival curve showed that CPSF6 expression was increased in patients with advanced non-small cell lung cancer,and progression-free survival and overall survival were decreased.Univariate and multivariate analysis showed that the expression level of CPSF6 protein,lymph node metastasis and clinical stage could be used as independent prognostic factors for patients with advanced non-small cell lung cancer(P＜0.05).Conclusion CPSF6 is highly expressed in advanced non-small cell lung cancer tissues and may play an important role in its prognosis.
Cleavage and polyadenylation specific factor 6Non-small cell lung cancerImmunohistochemistry
万方数据
维普
