Meta-analysis of the Diagnostic Value of Metagenomic Next-generation Sequencing Technology for Tuberculosis
Objective To evaluate the diagnostic value of metagenomic next-generation sequencing technology for tuberculosis using Meta-analysis.Methods PubMed MEDLINE,Ovid MEDLINE,Web of Science,Cochrane Library,EMbase,Scopus,CNKI,Wanfang,and VIP databases were searched for relevant literature on the application of metagenomic next-generation sequencing technology in the diagnosis of tuberculosis.The search time was from the establishment of the database to October 31,2021.Two researchers independently performed literature search,screening,data extraction,and quality evaluation.MetaDISc 1.4 software was used to test the threshold effect and heterogeneity,and the data were combined and analyzed.StataMP 16 software was used to draw Deek's funnel plot to evaluate publication bias.Results A total of 623 papers were retrieved.According to inclusion and exclusion criteria,14 studies involving 2159 samples,including 964 tuberculosis samples.Meta-analysis showed that the pooled sensitivity of metagenomic next-generation sequencing technology for the diagnosis of tuberculosis was 0.600(95％CI:0.568-0.631),the pooled specificity was 0.985(95％CI:0.976-0.991),the pooled positive likelihood ratio was 30.513(95％CI:18.536-50.226),the pooled negative likelihood ratio was 0.414(95％CI:0.357-0.481),the pooled diagnostic odds ratio was 77.408(95％CI:46.090-130.010),the pooled area under the curve was 0.9680 and the pooled Q*-value was 0.9167.Deek's funnel plot suggested no publication bias(P＞0.05).Conclusion The metagenomic next-generation sequencing technology has high diagnostic value for tuberculosis,and can be used as an effective method for rapid diagnosis.
万方数据
维普
