Pharmacological Mechanism of Qingdai in the Treatment of Ulcerative Colitis by Bioinformatics Methods
Objective To explore the pharmacological mechanism of Qingdai in the treatment of ulcerative colitis by network pharmacology.Methods The main chemical components and effective targets of Qingdai were obtained through the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP)database.The effective active components and potential targets were screened according to the pharmacokinetic parameters.The potential therapeutic targets of ulcerative colitis were obtained through GeneCards,OMIM,TTD and DRUGBANK databases,and the co-targets were obtained by making Venn diagram.String database was used for protein interaction analysis,PPI network diagram was constructed to mine potential protein function modules,and GO and KEGG enrichment analysis were performed.The"drug component-target-pathway"network diagram was constructed by Cytoscape 3.7.2 software and data analysis was performed.Results A total of 64 Qingdai component targets and 1599 ulcerative colitis-related targets were screened,and 32 drug-disease co-targets were obtained,which were enriched in biological functions such as cytokine receptor binding,DN A-binding transcription factor binding,and heme binding,as well as lipid and atherosclerosis(lipid and atherosclerosis),hepatitis B(hepatitis B),EB virus infection(epstein-bar virus infection)and other disease-related signaling pathways.Conclusion Indirubin is the main active ingredient of Qingdai in the treatment of ulcerative colitis,mainly by regulating RELA,TNF,IKBKB,CASP3,MAPK14,CASP8 and other targets,inhibiting cellular inflammatory response,improving intestinal epithelial cell function,so as to achieve the purpose of treating ulcerative colitis.
万方数据
维普
