Mechanism of Action of Dispsaci Radix in the Treatment of Rheumatoid Arthritis Based on Network Pharmacology
Objective To investigate the active ingredients and mechanism of dispsaci radix in the treatment of rheumatoid arthritis(RA)based on network pharmacology.Methods The drug compounds were screened by Herb,Batman-TCM database,and literature reports,the potential targets of the main active ingredients were screened by SwissTargetPrediction database,and the disease-related targets were screened by Gene Cards,OMIM,and TTD databases.The online mapping tool Venny was used to obtain the common targets of dispsaci radix and RA related targets.The protein-protein interaction(PPI)network was constructed by String,and the key target proteins were screened by Cytoscape software.The intersection targets were imported into the DAVID database for GO function and KEGG pathway enrichment analysis,and Cytoscape software was used to construct a live disease-pathway-target-drug interaction network.Results After screening,11 components and 178 potential targets of himalayan teasel root were obtained,including 1032 RA disease-related targets and 52 common targets of dispsaci radix-RA.Among them,VEGFA,EGFR and 1L-2 were the three target genes with the largest betweenness in the PPI network.GO functional analysis showed that the therapeutic effect of himalayan teasel root on RA was mainly related to the positive regulation of smooth muscle cell proliferation,the positive regulation of cell migration,the negative regulation of gene expression,extracellular space,cytoplasm,plasma membrane,endopeptidase activity,enzyme binding,zinc ion binding and so on.KEGG pathway enrichment analysis showed that it involved cancer pathway,calcium signaling pathway,blood lipid and atherosclerosis pathway,Ras signaling pathway,Rapl signaling pathway,chemical carcinogenesis-receptor activation pathway and other signaling pathways.Conclusion Dispsaci radix may treat RA by regulating VEGFA,EGFR,IL-2 and other targets,inhibiting inflammatory response and regulating immune function.
万方数据
维普
