Study on the Cryopreservation and Autologous Transplantation Technique of Mouse Ovarian Tissues
Objective To verify the safety and efficacy of ovarian cryopreservation and transplantation technique,and lay a clinical foundation for the protection of female fertility.Methods Totally 30 healthy sexually mature adult mice were selected and divided into study group A,study group B and control group,with 10 mice in each group.The mice in study groups were anesthetized and then had the right ovaries removed,buffered in a M2 medium containing 20％HAS,and then transferred to cryoprotectant for freezing.The ovaries were resuscitated 7 days later,and the right ovaries were transplanted to the position of left ovaries(the left ovaries had been removed).From the 5th day after transplantation,three groups received exfoliative vaginal cytology on a daily basis.On the 10th days after surgery,the mice in study group A were caged with male mice of the same strain,and their ovaries were removed to observe morphology on the 25th day of co-caging.The mice in study group B had their ovaries removed to observe histology on the 15th day of transplantation.Results The mice in the control group had a complete and regular oestrous cycle.In study group A and study group B,8 and 7 animals restored estrous cycles,respectively.After transplantation,some mice showed changes in ovarian volume,10％of the ovaries disappeared,40％atrophied and 50％didn't change.On the 15th day of transplantation,a lot of primordial follicles were visible on ovarian sections.On the 25th day of transplantation,the number of follicles on ovarian sections fell,and it was hard to find primordial follicles,and only secondary follicles,mature follicles and corpus luteum were visible.The density of follicles on the sections of transplanted ovaries was lower than that in normal ovaries.On the 22nd day of co-caging,two mice in study group A were pregnant and delivered 3 mice and 1 mouse respectively,the number of litters was lower than that of the control group.Conclusion It is feasible to perform cryopreservation and autologous orthotopic transplantation on the ovarian tissues of mice.
OvaryCryopreservationTransplantationProtection of fertility
万方数据
维普
