Study on Subchronic Toxicity of Millettia Speciosa
Objective To provide experimental basis for safety evaluation of Millettia speciosa by conducting subchronic toxicity test.Methods A total of 80 SPF SD rats(sex in half)were divided into low,medium and high dose groups[0.86、2.58、7.73 g/(kg·bw)]and a negative control group.Rats in the dose groups were orally administered the corresponding doses of millettia speciosa,while rats in the negative control group were given purified water.The oral gavage lasted for 90 days consecutively.At the end of the experiment,blood samples were collected from rats anesthetized by intraperitoneal injection for blood indexes analyses.Major organs were harvested and weighed to calculate organ-to-body weight ratios.Histopathological examinations were also performed on organ tissues.Results The growth and development of the animals in each dose group were good.There was no significant difference in body weight,food intake,food utilization rate,blood routine,blood clotting,blood biochemical index,organ weight,and viscera/body ratio index between each dose group and the negative control group(P＞0.05).No pathological changes related to the test samples were observed by histopathological observation.Conclusion Under the conditions of this experiment,the no observed adverse effect level(NOAEL)of the test sample Millettia speciosa on female and male rats is determined to be 75.00 g/(kg·bw),and no subchronic toxicity is observed.
万方数据
维普
