摘要
目的 初步从抗炎和抗氧化应激角度探讨表儿茶素对放射性口腔炎小鼠的保护作用.方法 将30只雄性C57BL/6J小鼠随机分为空白对照组、模型组和表儿茶素组,采用单次照射17 Gy X射线方法建立小鼠口腔炎模型.其中表儿茶素组于造模后连续7 d灌胃给予表儿茶素30 mg/kg,其余两组灌胃给予等量生理盐水.各组小鼠进行口腔炎症评分,HE染色法观察口腔黏膜病理变化,酶联免疫吸附法检测血清肿瘤坏死因子α(TNF-α)、白介素1β(IL-1β)和白介素6(IL-6)水平;化学发光法检测血清超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)的活性以及丙二醛(MDA)水平.结果 与空白对照组比较,模型组小鼠口腔炎症评分、血清TNF-α、IL-1β、IL-6和MDA水平升高(P＜0.05),血清SOD、GSH-Px和CAT活性降低(P＜0.05);与模型组比较,表儿茶素组口腔炎症评分、血清TNF-α、IL-1β、IL-6和MDA水平降低(P＜0.05),血清SOD、GSH-Px和CAT活性升高(P＜0.05).结论 表儿茶素对放射性口腔炎小鼠具有较好的保护作用,其机制可能与抑制炎症和抗氧化损伤有关.
Abstract
Objective To investigate the protective effect of epicatechin on radiation stomatitis mice from the perspective of anti-inflammatory and anti-oxidative stress.Methods Thirty male C57 BL/6J mice were randomly divided into blank control group,model group and epicatechin group.The mouse model of stomatitis was established by single irradiation of 17 Gy X-ray.The epicatechin group was given epicatechin 30 mg/kg by gavage for 7 consecutive days after modeling,and the other two groups were given the same amount of normal saline by gavage.The oral inflammation score of mice in each group was evaluated.The pathological changes of oral mucosa were observed by HE staining.The levels of serum tumor necrosis factor-α(TNF-α),interleukin-1 β(IL-1 β)and interleukin-6(IL-6)were detected by enzyme-linked immunosorbent assay.And the activities of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)and catalase(CAT)and the level of malondialdehyde(MDA)in serum were detected by chemiluminescence.Results Compared with blank control group,the oral inflammation score,serum levels of TNF-α,IL-1β,IL-6 and MDA in model group were increased(P＜0.05),while the serum activities of SOD,GSH-Px and CAT were decreased(P＜0.05).Compared with model group,the oral inflammation score,serum levels of TNF-α,IL-1β,IL-6 and MDA in epicatechin group were decreased(P＜0.05),while the serum activities of SOD,GSH-Px and CAT were increased(P＜0.05).Conclusion Epicatechin has a good protective effect on mice with radiation stomatitis,and its mechanism may be related to the inhibition of inflammation and antioxidant damage.
基金项目
深圳市龙华区医疗卫生机构区级科研项目(2021021)
维普
万方数据