摘要
目的 探讨差异表达基因CDCA8及CDC20在肾母细胞瘤中的表达,以及与肾母细胞瘤患者预后及免疫细胞浸润的关系.方法 登录并下载GEO数据库及Target数据库中与肾母细胞瘤相关的基因表达谱数据;利用R语言筛选出差异表达基因,筛选出与肾母细胞瘤预后有关的基因;利用R语言中的CIBERSORT计算方法计算出肾母细胞瘤中免疫细胞浸润的表达;利用SPSS分析肾母细胞瘤预后有关的基因与免疫细胞浸润的相关性.结果 GEO表达谱数据GSE66405、GSE73209、GSE11151筛选出共同差异表达基因1260个,其中高表达398个,低表达862个;蛋白互作网络筛选出15个核心蛋白,包括CDK1、CCNA2、TOP2A、CCNB2、KIF11、BUB1、BUB1B、ASPM、NUSAP1、CDC20、CDCA8、KIF20A、DLGAP5、TPX2、CENPF,其中 CDCA8 及 CDC20 与肾母细胞瘤患者预后有关;T cells CD4 memory activated(P=0.026)、T cells follicular helper(P=0.031)、Macrophages M0(P=0.005)、Dendritic cells activated(P=0.025)的表达高于正常对照组,而 Plasma cells(P=0.023)、T cells CD4 memory resting(P=0.002)、NK cells resting(P=0.045)、Monocytes(P=0.013)在肾母细胞瘤中低表达.相关性分析显示,CDCA8与6个免疫细胞浸润具有相关性,CDC20与5个免疫细胞浸润具有相关性.结论 CDCA8及CDC20有可能成为肾母细胞瘤患者治疗的新潜在靶点.
Abstract
Objective To investigate the expression of differentially expressed genes CDCA8 and CDC20 in nephroblastoma and their relationship with prognosis and immune cell infiltration in patients with nephroblastoma.Methods The gene expression profile data related to nephroblastoma in GEO database and Target database were logged in and downloaded.The differentially expressed genes were screened by R language,and the genes related to the prognosis of nephroblastoma were screened.The expression of immune cell infiltration in nephroblastoma was calculated by CIBERSORT calculation method in R language.SPSS was used to analyze the correlation between genes related to the prognosis of nephroblastoma and immune cell infiltration.Results A total of 1260 common differentially expressed genes were screened out from GEO expression profile data GSE66405,GSE73209 and GSE11151,of which 398 were highly expressed and 862 were lowly expressed.The protein interaction network screened 15 core proteins,including CDK1,CCNA2,TOP2 A,CCNB2,KIF11,BUB1,BUB1B,ASPM,NUSAP1,CDC20,CDCA8,KIF20 A,DLGAP5,TPX2,CENPF,among which CDCA8 and CDC20 were related to the prognosis of nephroblastoma patients.The expression of T cells CD4 memory activated(P=0.026),T cells follicular helper(P=0.031),Macrophages M0(P=0.005)and Dendritic cells activated(P=0.025)in nephroblastoma was higher than that in normal control group.Plasma cells(P=0.023),T cells CD4 memory resting(P=0.002),NK cells resting(P=0.045)and Monocytes(P=0.013)were lowly expressed in nephroblastoma.Correlation analysis showed that CDCA8 was correlated with 6 immune cell infiltration,while CDC20 was correlated with 5 immune cell infiltration.Conclusion CDCA8 and CDC20 may be new potential targets for the treatment of patients with nephroblastoma.
基金项目
广西卫生健康委自筹经费科研课题(Z-L20220850)
"登峰计划"高原学科建设项目(DF20244427)
维普
万方数据