Effect of hsa-miR-155-5p on the Immune Microenvironment of Hepatocellular Carcinoma by Regulating Central Memory T Cells Helper T Cells Through PTEN Based on the Bioinformatics Method
Objective To predict the target genes of human miR-155-5p by bioinformatics methods,and to further explore its function in hepatocellular carcinoma(HCC).Methods The target genes of hsa-miR-155-5p were screened by starBase database,and functional enrichment analysis was performed by DAVID and Reactome databases.Protein-protein interaction network was used to analyze the relationship between target genes,and cytohubba plug-in was used to obtain key genes.Kaplan-Meier survival analysis was used to further screen key genes,ssGSEA algorithm was used to analyze the immune infiltration of HCC,and Spearman correlation coefficient was used to analyze the correlation between key genes and immune infiltration.Results A total of 1493 hsa-miR-155-5p target genes were screened.GO enrichment analysis of target genes focused on the regulation of transcription,protein catabolic process and cell cycle regulation.The KEGG biological pathway was mainly enriched in AMPK signaling pathway,PI3K-Akt signaling pathway and FoxO signaling pathway,as well as endometrial cancer,pancreatic cancer and hepatocellular carcinoma.A total of 30 key genes were obtained by protein interaction network,and 10 key genes such as GSK3B,PTEN,PIK3R1,EGFR,CCND1,SMAD2,PIK3CA,RPS6KB1,KRAS and SOS1 were obtained by intersection with Hepatocellular carcinoma signaling pathway genes.Survival analysis showed that the prognosis of HCC patients with high expression of PTEN was better than that of patients with low expression.The expression of PTEN was positively correlated with the immune infiltration of central memory T cells and helper T cells.Conclusion hsa-miR-155-5p regulates central memory T cells and helper T cells through the downstream target gene PTEN,thereby affecting the immune infiltration microenvironment of HCC,which may provide help for the exploration of HCC pathogenesis and clinical treatment.
Hepatocellular carcinomahsa-miR-155-5pBioinformaticsPTENCentral memory T cellsHelper T cells
国家科技期刊平台
NSTL
万方数据
维普
