氯化钴模拟低氧条件下红景天苷对小鼠成骨细胞的调节作用
Regulatory Effect of Salidroside on Mouse Osteoblasts Under Hypoxic Conditions Simulated by Cobalt Chloride
罗婷 1祁琳 2李杰 1薛文舒 3田晨晨 3曹清文 3王越3
作者信息
- 1. 天津医科大学总医院口腔科,天津 300052
- 2. 武警特色医学中心药剂科,天津 300162
- 3. 天津中医药大学中西医结合学院,天津 301617
- 折叠
摘要
目的 研究氯化钴(CoCl2)模拟的低氧条件下红景天苷(SAL)对小鼠成骨细胞的调节作用.方法 用CoCl2模拟低氧条件(1％O2),建立体外低氧模型.采用MTS法研究低氧条件下不同浓度SAL对MC3T3-E1细胞增殖的影响,磷酸苯二钠法研究低氧条件下SAL对MC3T3-E1细胞分化的影响,实时荧光定量PCR(RT-qPCR)、Western blot及ELISA技术检测低氧诱导因子-1α(HIF-1α)、下游靶基因血管内皮生长因子(VEGF)mRNA及蛋白表达水平.结果 确定用0.5 mmol/L的CoCl2来模拟低氧(1％O2)环境.低氧可明显抑制MC3T3-E1细胞的增殖及分化.经SAL预处理后,可显著促进MC3T3-E1细胞的增殖及分化,其中SAL(10 nmol/L)可显著上调HIF-1α和VEGF的mRNA表达水平及HIF-1α的蛋白表达水平,并下调VEGF蛋白的表达.结论 低氧条件下SAL促进小鼠成骨细胞增殖与分化.其具体机制有待进一步研究,可能与HIF-1α/VEGF信号通路有关.
Abstract
Objective To study the regulatory effect of salidroside(SAL)on mouse osteoblasts under hypoxic conditions simulated by cobalt chloride(CoCl2).Methods CoCl2 was used to simulate hypoxic conditions(1％02)to establish an in vitro hypoxic model.MTS method was used to study the effect of different concentrations of SAL on the proliferation of MC3T3-E1 cells under hypoxic conditions.The effect of SAL on the differentiation of MC3T3-E1 cells under hypoxic conditions was studied by disodium phenyl phosphate method.Real-time fluorescence quantitative PCR(RT-qPCR),Western blot and ELISA were used to detect the mRNA and protein expression levels of hypoxia-inducible factor-1α(HIF-1α)and downs.Results 0.5 mmol/L CoCl2 was used to simulate the hypoxic(1％02)environment.Hypoxia could significantly inhibit the proliferation and differentiation of MC3T3-E1 cells.After pretreatment with SAL,the proliferation and differentiation of MC3T3-E1 cells were significantly promoted.SAL(10 nmol/L)significantly up-regulated the mRNA expression levels of HIF-1α and VEGF and the protein expression level of HIF-1α,and down-regulated the expression of VEGF protein.Conclusion SAL promotes the proliferation and differentiation of mouse osteoblasts under hypoxia.The specific mechanism needs to be further studied,which may be related to the HIF-1α/VEGF signaling pathway.
关键词
红景天苷/成骨细胞/增殖/分化/骨结合/HIF-1α/VEGF通路Key words
Salidroside/Osteoblasts/Proliferation/Differentiation/Osseointegration/HIF-1α/VEGF pathway引用本文复制引用
基金项目
天津伊特生命科学研发有限公司项目(HX2023-7)
天津市自然科学基金重点项目(18JCZD-JC33200)
天津中医药大学研究生创新基金(ZXY-CXLX202119)
天津中医药大学研究生创新基金(ZXYCXLX202213)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据