Motion Tracking Analysis of High-content Non-fluorescent Labelling in Living Cells
Cell movement is closely related to a variety of cell behaviors and disease treatment.Real-time cell tracking and analysis can provide experimental basis for revealing cell behavior,cell movement rules,and disease mechanism.At present,the conventional experimental methods of cell tracking are mostly indirect analysis of cell migration from the group level,which cannot track the changes of parameters such as morphology and motion dynamics of single cells during migration.Especially for non-fluorescent labelling in living cells,the tracking analysis of conventional bright field or phase difference images has problems such as low contrast between the background and cells in the image and discontinuous cell boundaries.It is difficult to achieve accurate identification and resolution of cell morphology and automatic tracking analysis.The high-content imaging system equipment combines the live cell imaging module and multi-parameter high-content analysis software,which can realize fast imaging of unlabeled live cells and automatic segmentation,recognition and tracking analysis.This paper summarizes the application of the label-free cell analysis module of the high-content imaging system in in vitro cell movement tracking experiments.
High-content imaging analysisLabel-free living cellsCell tracking
万方数据
维普
