Expression and Clinical Significance of Pyroptosisin Peripheral Blood and Placental Tissues of Patients with Placenta Implantation
Objective To investigate the expression of inflammatory factors in peripheral blood of patients with placenta implantation and the expression of pyroptosis factors in placental tissues and their clinical significance.Methods From June 2021 to January 2023,30 pregnant women diagnosed with placenta implantation in the obstetrics department of our hospital were collected as the placenta implantation group,and 60 normal pregnant women during pregnancy were collected as the normal group.The clinical data of the two groups were analyzed.The expression of TNF-α,IL-1β and IL-18 was detected by ELISA.The histopathology of placenta implantation was observed by HE staining.The expression of Caspase-1,GSDMD and NLRP3 protein and mRNA in placenta of two groups was detected by IHC and qPCR.Results The expression levels of TNF-α,IL-1 βand IL-18 in peripheral blood of pregnant women in the placenta implantation group were higher than those in the normal group,and the differences were statistically significant(P＜0.05).There was no decidua in the uterine basal tissue of patients with placenta implantation,and the chorionic villi were directly adjacent to the myometrium fibers.The expression of Caspase-1 and NLRP3 protein and mRNA in placenta tissue of placenta implantation group was higher than that of normal group,and the expression of GSDMD protein and mRNA was lower than that of normal group(P＜0.05).Conclusion The expression of pyroptosis is increased in the pathogenesis of placental implantation.
万方数据
维普
