Experimental Study on the Effect of Cold Pathogen on Lumbar Disc Herniation in Rats
Objective To investigate the effect of cold pathogen on rat model of lumbar disc herniation.Methods Thirty SD rats were randomly divided into experimental group,control group and blank group according to the random number table method,with 10 rats in each group.The experimental group and the control group were transplanted with autologous nucleus pulposus to L4 and L5 nerve roots to establish a rat model of lumbar disc herniation.The blank group was not treated.The experimental group and the blank group were placed at 12:00-16:00 every day for 7 consecutive days.The rats were raised in an artificial climate box with a temperature of(4±0.5)℃ and a humidity of 50％±2％for 4 h,and the rest of the time was raised at room temperature.The control group was placed in a room temperature of(25±0.5)℃ and a humidity of 50％±2％for standardized feeding for 1 week.The quality of life,the content of PLA2 in nucleus pulposus,the content of serum inflammatory factors TNF-α and IL-6,and the pathological section of intervertebral disc were observed in the three groups.Results After the completion of the modeling,all the three groups of rats survived without death,paralysis,and infection.On the third day of the experiment,the three groups of rats had obvious symptoms,and the neurological function scores were all above grade 3.On the 7th and 14th day of the experiment,the neurological function scores of the experimental group were higher than those of the control group and the blank group(P＜0.05),there was no significant difference in neurological function score between the 14th day and the 7th day in the experimental group(P＞0.05),while the neurological function scores of the control group and the blank group on the 14th and 7th days of the experiment were statistically significant(P＜0.05).The levels of serum TNF-α and IL-6 on the 14th day of the experiment were lower than those on the 7th day of the experiment,but the levels of serum TNF-α and IL-6 on the 7th and 14th days of the experiment in the experimental group were higher than those in the control group and the blank group(P＜0.05).The content of PLA2 in the nucleus pulposus of rats on the 14th day of the three groups was lower than that on the 7th day of the experiment,but the content of PLA2 in the nucleus pulposus of rats on the 7th and 14th days of the experiment in the experimental group was higher than that in the control group and the blank group(P＜0.05).Conclusion The cold pathogen acting on the rat model of lumbar disc herniation can reduce the quality of life of rats,increase the content of PLA2 in the nucleus pulposus and the expression of serum inflammatory factors TNF-α and IL-6,which is an important pathogenic factor of lumbar disc herniation.
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