Experimental Study of IL-13Ra2 as an Independent Biomarker in the Diagnosis of Pulmonary Hypertension
Objective To investigate the role of IL-13RA2 in the diagnosis and evaluation of pulmonary hypertension in rats.Methods Nine specific pathogen free(SPF)male Sprague-Dawley rats aged 6-8 weeks were divided into control group(4 rats)and pulmonary hypertension group(5 rats)by random number table method.The rat model of pulmonary hypertension was constructed by monocrotaline,and echocardiography was performed.At the same time,the heart,lung,pulmonary artery and other tissues of the rats were collected for H & E staining and immunohistochemistry.Results Echocardiography showed that the thickness of ventricular septum in the pulmonary hypertension group was thicker than before,and the heart volume was larger than before.The pulmonary artery pressure of the pulmonary hypertension group was higher than that of the control group,the thickness of the ventricular septum was thicker than that of the control group,and the inner diameter of the right ventricle was longer than that of the control group,but the difference was not statistically significant(P＞0.05).The heart weight and heart body index of the pulmonary hypertension group were higher than those of the control group,and the right ventricular hypertrophy index was not significantly higher than that of the control group.HE staining showed that the alveolar wall of the rats in the pulmonary hypertension group was thickened,the inflammatory cells were infiltrated,the alveolar results were obviously deformed,the pulmonary artery wall was thickened,the smooth muscle was proliferated,the lumen was narrowed or even occluded,the arrangement of myocardial cells was obviously disordered,the gap between myocardial cells was narrowed,and the myocardial cells were hypertrophic.The results of immunohistochemistry showed that the expression of IL-13Ra2 in lung tissue,pulmonary artery and myocardium of rats in pulmonary hypertension group was significantly increased.Conclusion IL-13Ra2 can be used as an independent biomarker in the diagnosis of patients with pulmonary hypertension.
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