Effect of Different Immunoassays on the Detection of Serum Markers of Hepatitis B Virus Infection
Objective To study the effect of different immune test methods on the test effect of serum markers of hepatitis B virus infection.Methods A total of 60 patients with suspected hepatitis B admitted to Anyi County People's Hospital from June 2020 to June 2023 were selected as the research objects.The serum markers[hepatitis B surface antigen(HBsAg),hepatitis B surface antibody(HBsAb),hepatitis B e antigen(HBeAg),hepatitis B e antibody(HBeAb),and hepatitis B core antibody(HBcAb)]were detected by chemiluminescence immunoassay(CLIA)and enzyme-linked immunosorbent assay(ELISA),respectively.The diagnostic efficacy of the two methods for hepatitis B and the positive detection rate of hepatitis B serum markers were compared.The diagnostic accuracy of different hepatitis B types was statistically analyzed.Results The sensitivity,accuracy and specificity of CLIA in the diagnosis of hepatitis B were higher than those of ELISA(P＜0.05).The positive detection rates of HBsAg,HBsAb and HBeAg by CLIA were higher than those by ELISA(P＜0.05),but there was no significant difference in the positive detection rates of HBcAb and HBeAb between CLIA and ELISA(P＞0.05).The diagnostic accuracy of CLIA was higher than that of ELISA(P＜0.05),but there was no significant difference in the diagnostic accuracy between between CLIA and ELISA(P＞0.05).Conclusion CLIA and ELISA have definite application value in the diagnosis of hepatitis B.The diagnostic efficiency of CLIA is higher,and its detection effect on serum markers is more ideal.
Hepatitis BSerum markersChemiluminescence immunoassayEnzyme linked immunosorbent assayHepatitis B surface antigen
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维普
