Effect of Three Kinds of Vacuum Blood Collection Specimens on the Detection of Total Protein and Albumin Results
Objective To study the effects of three kinds of vacuum blood collection tubes on the results of total protein and albumin detection by different methods.Methods Venous blood samples of 80 healthy subjects were randomly selected and injected into ordinary additive-free glass test tube(A tube),test tube containing coagulant+separation gel(B tube)and heparin anticoagulant test tube(C tube).At the same time,total protein was detected by biuret method,and albumin was detected by bromocresol green method/immunoturbidimetric method.The results of the three tubes were statistically analyzed to compare the differences,so as to measure the interference degree of different intravascular additives on the detection of total protein by biuret method and albumin by bromocresol green method/immunoturbidimetric method.Results The comparison experiments of different vacuum blood collection tubes confirmed that the results of total protein detection by biuret method in ordinary additive-free glass test tube(A tube)and coagulant test tube(B tube)were lower than those in heparin anticoagulant test tube(C tube);the results of albumin detection by bromocresol green method in coagulant test tube(B tube)and heparin anticoagulant test tube(C tube)were lower than those of ordinary additive-free glass test tube(A tube),and the difference was statistically significant(P＜0.05).There was no significant difference in the results of albumin detected by immunoturbidimetry among the three tubes(P＞0.05).Conclusion The detection of total protein and albumin is more affected by interference factors such as intravascular additives.For the samples with abnormal levels of total protein and albumin,it is recommended to use the method of replacing the blood collection tube or less interference factors such as immunoturbidimetry to obtain reliable results.
Vacuum blood collection tubeHeparinCoagulantTotal proteinHuman serum albumin
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