Analysis of Respiratory Tract Microecology in Patients with Infectious Pulmonary Nodules
Objective To analyze the composition and changes of respiratory microecological components in patients with infectious pulmonary nodules.Methods A total of 53 patients with pulmonary sarcoidosis diagnosed and treated in the Department of Respiratory and Critical Care Medicine of our hospital from June 2020 to December 2022 were collected.According to the clinical diagnosis,they were divided into infection group(22 patients)and non-infection group(31 patients).Bronchoalveolar lavage fluid was collected for metagenomic sequencing(mNGS)to detect respiratory microorganisms.The Alpha diversity of respiratory microorganisms and the ROC and PRC curves of each index were compared between the two groups.Results The Sobs index,Chao1 index and Shannon index of the infected group were higher than those of the non-infected group,while the Simpson index was lower than that of the non-infected group,but the difference were not statistically significant(P＞0.05).The ACE index of the infected group was higher than that of the non-infected group,and the difference was statistically significant(P＜0.05).The non-infected group was dominated by G-negative and virus,while the infected group was dominated by G-positive bacteria,and the difference was statistically significant(P＜0.05).The error rates of Sobs,Chao1 and Simpson indices were higher than 50％,and only ACE and Shannon indices were lower than 50％.The accuracy,specificity,sensitivity and accuracy of ACE and Shannon index in the diagnosis of infectious pulmonary nodules were higher than those of other indexes.The AUC of Sobs,Chao1,AEC,Shannon and Simpson index ROC were 0.396,0.394,0.569,0.582 and 0.449,respectively.The AUC of PRC was 0.476,0.474,0.562,0.647 and 0.511,respectively.The AUC of combined detection of ROC and PRC was 0.482 and 0.520.Conclusion Respiratory tract microecology is related to patients with infectious pulmonary nodules.The analysis of Alpha diversity of respiratory tract microorganisms can be used for clinical diagnosis and treatment of patients with infectious pulmonary nodules,which has certain application value.
万方数据
维普
