Value of Different Detection Methods in the Screening and Diagnosis of Syphilis Specific Antibodies
Objective To study the value of different detection methods in the screening and diagnosis of syphilis-specific antibodies.Methods A total of 60 patients with syphilis diagnosed in our hospital from January 2020 to April 2023 were selected as the research objects.Electrochemiluminescence immunoassay(ECLIA),chemiluminescence immunoassay(CLIA)and enzyme-linked immunosorbent assay(ELISA)were used to detect syphilis-specific antibodies.The colloidal gold method of rapid treponema pallidum antibody(gold standard method)was used as the standard to compare the results of different detection methods,diagnostic efficacy(sensitivity,specificity,accuracy,positive predictive value,negative predictive value),and consistency with the results of gold standard method.Results When ECLIA and CLIA S/CO＜l,all specimens were negative by gold standard method.When S/CO＜1 by ELISA,2 samples were positive by gold standard method.When S/CO≥5 by ECLIA,S/CO≥7 by CLIA and S/CO≥7 by ELISA,the positive rate ofgold standard method was 100.00％.There was no significant difference in the sensitivity,specificity,accuracy,positive predictive value and negative predictive value of ECLIA,CLIA and ELISA in the detection of syphilis specific antibody(P＞0.05).There was no significant difference in the consistency between the results of syphilis specific antibody detection by ECLIA,CLIA,ELISA and gold standard method(P＞0.05).ROC curve analysis showed that there was no significant difference in AUC area between ECLIA and CLIA in the diagnosis of syphilis(P＞0.05),while the AUC area of CLIA and ECLIA in the diagnosis of syphilis was greater than that of ELISA(P＜0.05).Conclusion When ECLIA,CLIA and ELISA are used to screen syphilis,S/CO is less than 5,7 and 7,respectively,gold standard method should be used for verification to reduce misdiagnosis.However,the diagnostic efficiency of ECLIA and CLIA was higher than that of ELISA.
Syphilis specific antibodySyphilisScreening functionDiagnostic efficacy
万方数据
维普
