Shunt Value of HPV E6/E7 mRNA Detection in HPV16/18 DNA Positive Patients
Objective To investigate the value of HPV E6/E7 mRNA in the shunt of HPV16/18 DNA positive patients.Methods A total of 127 cases of HPV16/18 DNA positive nucleic acid samples were collected,and HPV E6/E7 mRNA was detected.The results were statistically analyzed in combination with the results of thinprepcytologic test(TCT)and pathological diagnosis.Results ①The HPV E6/E7 mRNA was detected by mainly single infection,accounting for 81.93％.The age distribution showed a bimodal shape.②The total positive rate of HPV E6/E7 mRNA was 65.35％.With the increase of cervical lesions,the positive rate of HPV E6/E7 mRNA increased,while the positive rate of LSIL group and inflammation group,HSIL+group and inflammation group was statistically significant(P＜0.05).③As the HPV 16/18 DNA positivity triage test,Cytology at the ASC-US+threshold could reduce colposcopy by 65.35％,but missed diagnosis of HSIL+by 44.83％;whereas HPVE6/E7 mRNA could reduce the colposcopy by 34.65％,and missed diagnosis of HSIL+by 10.34％.As a triage test in HPV 16/18 DNA positivity patients,the missed diagnosis rate of HPV E6/E7 mRNA was lower than that of TCT(P＜0.05).④As a triage test,in patients with HPV16/18 DNA positive and cytological grades ≥ASC-US,E6/E7 mRNA could reduce the colposcopy by 11.36％and none missed diagnosis of HSIL+.Conclusion TCT combined with HPV E6/E7 mRNA for synchronous shunt in HPV16/18 DNA positive patients can reduce colposcopy and the risk of missed diagnosis of HS1L+in patients with ≥ASC-US.
万方数据
维普
